Delivery of erythropoietin-loaded PLGA nanospheres to central nervous system via blood-brain barrier transport : Therapy for neonatal hypoxic-ischemic encephalopathy

• Project/Area Number: 25860131
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Mukogawa Women's University
• Principal Investigator: HAZEKAWA Mai 武庫川女子大学, 薬学部, 助教 (10509186)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: エリスロポエチン / ナノスフェア / 自己修復 / 脳性小児麻痺 / PLGA / 脳移行性 / 徐放性製剤 / 初期バースト / 表面電荷 / 初期放出

Outline of Final Research Achievements

The purpose of this study was development of erythropoietin-loaded poly (lactide-co-glycolide) nanospheres for therapy of neonatal hypoxic-ischemic encephalopathy. Erythropoietin was encapsulated by poly as slow release formulation to reduce side effects. Furthermore, this particle showed low initial burst and high blood brain barrier permeability when this particle was prepared at glass transition temparatures of PLGA. These results were attributed to improvement the structure of surface in particle and decrease of specific surface area in particle. It was suggested that this particle might be useful in therapy for neonatal hypoxic-ischemic encephalopathy.

Research Products

• [Journal Article] The angiogenic effect of ONO-1301, a novel long-acting prostacyclin agonist loaded in PLGA microspheres prepared using different molecular weights of PLGA, in a murine sponge model (2014)
  • Author(s): Hazekawa M, Morihata K, Yoshida M, Sakai Y, Uchida T.
  • Journal Title: Drug. Dev. Ind. Pharm. Volume: 40 Issue: 11 Pages: 1435-1442
  • DOI: 10.3109/03639045.2013.828220
  • Peer Reviewed
• [Presentation] 自己修復を利用したOVA含有PLGAマイクロスフェア製剤の初期バースト抑制効果 (2015)
  • Author(s): 櫨川舞、原口珠実、吉田都、内田享弘
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸サンボーホール（兵庫県神戸市）
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] PGI2アゴニスト含有PLGA自己修復マイクロスフェアの調製に関する検討－初期バーストに及ぼすガラス転移点の影響－ (2014)
  • Author(s): 櫨川舞、原口珠実、吉田都、内田享弘
  • Organizer: 第64回日本薬学会近畿支部大会
  • Place of Presentation: 京都薬科大学（京都府京都市）
  • Year and Date: 2014-10-11
• [Presentation] PGI2アゴニスト含有自己修復マイクロスフェアの調製と評価 (2014)
  • Author(s): 櫨川舞、原口珠実、吉田都、内田享弘
  • Organizer: 日本薬剤学会第29年会
  • Place of Presentation: 大宮ソニックシティビル（埼玉県さいたま市）
  • Year and Date: 2014-05-20 – 2014-05-22
• [Presentation] PGI2アゴニスト含有自己修復マイクロスフェアの調製とその評価 (2014)
  • Author(s): 櫨川舞、原口珠実、吉田都、内田享弘
  • Organizer: 日本薬剤学会第29年会
  • Place of Presentation: 大宮ソニックシティビル
• [Presentation] The angiogenesis effectof a novel long-acting prostacyclin agonist loaded-PLGA microspheres prepared using different molecular weight in murine sponge model (2013)
  • Author(s): Mai Hazekawa, Tamami Haraguchi, Miyako Yoshida, Takahiro Uchida
  • Organizer: 40th Annual Meeting & Exposition of the Cntrolled Release Society
  • Place of Presentation: Hawaii Convention Center