Functional analysis of microRNA in tendon/ligament formation
| Project/Area Number |
25860139
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ITO Yoshiaki 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (50511044)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | microRNA / 腱・靭帯 |
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| Outline of Final Research Achievements |
We performed taqman array analysis of the cells expressing Mkx, a tendon/ligament specific transcription factor, using E14.5 Mkx-Venus knock-in embryos. Then, miRNAs expressed in Mkx positive cells were analyzed the expression in Achilles tendon and skeletal muscle, and we identified 3 miRNAs which showed high expression in Achilles tendon. We developed a miRNA targets screening system using a reporter library, which inserted a whole cDNA sequence in the 3’UTR of a luciferase gene. Using this screening system, we identified 3 miRNA targets. We developed the generation of knockout mice using genome-engineering technique and generated and analyzed the knockout mice in which miRNA is highly expressed in tendon.
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Report
(3 results)
Research Products
(3 results)
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[Journal Article] Targeted gene deletion of miRNAs in mice by TALEN system.2013
Author(s)
Takada S, Sato T, Ito Y, Yamashita S, Kato T, Kawasumi M, Kanai-Azuma M, Igarashi A, Kato T, Tamano M, Amahara H.
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Journal Title
PLoS One.
Volume: 8
Issue: 10
Pages: e76004-e76004
DOI
Related Report
Peer Reviewed
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