| Project/Area Number |
25860139
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General anatomy (including histology/embryology)
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
ITO Yoshiaki 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (50511044)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | microRNA / 腱・靭帯 |
|---|
| Outline of Final Research Achievements |
We performed taqman array analysis of the cells expressing Mkx, a tendon/ligament specific transcription factor, using E14.5 Mkx-Venus knock-in embryos. Then, miRNAs expressed in Mkx positive cells were analyzed the expression in Achilles tendon and skeletal muscle, and we identified 3 miRNAs which showed high expression in Achilles tendon. We developed a miRNA targets screening system using a reporter library, which inserted a whole cDNA sequence in the 3’UTR of a luciferase gene. Using this screening system, we identified 3 miRNA targets. We developed the generation of knockout mice using genome-engineering technique and generated and analyzed the knockout mice in which miRNA is highly expressed in tendon.
|
