| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Phagocytosis is a fundamental pathway that enables cells to eliminate pathogens, and endogenous cell debris, and contribute to immunoprotection and the maintenance of tissue homeostasis. In this study, we found that Rit1 GTPase is recruited to the membrane of phagocytic cups during FcγR-mediated phagocytosis in macrophages. Live-cell imaging analysis revealed that Rit1 is transiently colocalized with PI(4,5)P2 and PI(3,4,5)P3 during early stage of phagosome formation. CRISPR/Cas9-mediated Rit1 knockout or the expression of GDP-locked mutant Rit1-S35N suppressed phagocytosis of IgG-opsonized erythrocytes. These data suggest that Rit1 is a crucial regulator of FcγR-mediated phagocytosis in macrophages.
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