Deciphering novel functions and regulatory mechanisms of anterior pituitary cells using 3 dimensional cell culture

• Project/Area Number: 25860147
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Jichi Medical University
• Principal Investigator: TSUKADA Takehiro 自治医科大学, 医学部, 講師 (50596210)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 下垂体前葉 / 濾胞星状細胞 / 3次元培養 / 細胞外マトリックス / コラーゲン / ラミニン / 組織構築 / TGFbeta

Outline of Final Research Achievements

Aim of this project is to decipher a role of folliculostellate cells in tissue formation of anterior pituitary gland. This project utilized a 3 dimensional cell culture of rat anterior pituitary cells established by our laboratory, and S100b-GFP transgenic rats that express GFP in folliclulostellate cells. This project found 1) folliculostellate cells are required for release of major basement membrane component, laminin, from goandotrophs, and 2) TGFb2 secreted from folliculostellate cells induces collagen synthesis of microvessel mural cells, pericytes. In conclusion, this project revealed a novel function of folliculostellate cells, namely, a regulation of extracellular matrix within anterior pituitary gland.

Research Products

• [Journal Article] Histochemical Analysis of Laminin α Chains in Diethylstilbestrol-Induced Prolactinoma in Rats (2015)
  • Author(s): Ramadhani D, Tofrizal A, Tsukada T, and Yashiro T
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 48 Issue: 2 Pages: 69-73
  • DOI: 10.1267/ahc.14067
  • NAID: 130005068299
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Isolation of dendritic-cell-like S100β-positive cells in rat anterior pituitary gland. (2014)
  • Author(s): Kotaro Horiguchi, Ken Fujiwara, Saishu Yoshida, Masashi Higuchi, Takehiro Tsukada, Naoko Kanno, Takashi Yashiro, Kozue Tateno, Shunji Osako, Takako Kato, Yukio Kato
  • Journal Title: Cell and Tissue Research Volume: (印刷中) Issue: 1 Pages: 301-308
  • DOI: 10.1007/s00441-014-1817-9
  • Peer Reviewed
• [Journal Article] Expression of chemokine CXCL10 in dendritic-cell-like S100β-positive cells in rat anterior pituitary gland (2014)
  • Author(s): Horiguchi K, Fujiwara K,他11名
  • Journal Title: Cell Tissue Res. Volume: 357 Issue: 3 Pages: 757-765
  • DOI: 10.1007/s00441-014-1864-2
  • Peer Reviewed
• [Journal Article] In situ hybridization analysis of the temporospatial expression of the midkine/pleiotrophin family in rat embryonic pituitary gland (2014)
  • Author(s): Fujiwara K,他9名
  • Journal Title: Cell Tissue Res. Volume: 357 Issue: 1 Pages: 337-344
  • DOI: 10.1007/s00441-014-1875-z
  • Peer Reviewed
• [Journal Article] Phosphorylation of Tropomodulin1 Contributes to the Regulation of Actin Filament Architecture in Cardiac Muscle (2014)
  • Author(s): Bliss KT, Tsukada T, Novak SM, Dorovkov MV, Shah S, Nworu C, Kostyukova AS, and Gregorio CC
  • Journal Title: FASEB J Volume: 28 Issue: 9 Pages: 3987-3995
  • DOI: 10.1096/fj.13-246009
  • Peer Reviewed
• [Journal Article] Changes in Laminin Chain Expression in Pre- and Postnatal Rat Pituitary Gland (2014)
  • Author(s): Ramadhani D, Tsukada T, Fujiwara K, Azuma M, Kikuchi M, Yashiro T
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 47 Issue: 5 Pages: 231-237
  • DOI: 10.1267/ahc.14031
  • NAID: 130004700244
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Folliculostellate Cells Are Required for Laminin Release from Gonadotrophs in Rat Anterior Pituitary (2014)
  • Author(s): Tsukada T, Fujiwara K, Horiguchi K, Azuma M, Ramadhani D, Tofrizal A, Batchuluun K, Maliza R, Syaidah R, Kikuchi M, Yashiro T
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 47 Issue: 5 Pages: 239-245
  • DOI: 10.1267/ahc.14036
  • NAID: 130004700245
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Expression of the heparin-binding growth factor midkine and its receptor, Ptprz1, in adult rat pituitary (2014)
  • Author(s): Fujiwara K, Horiguchi K, Maliza R, Tofrizal A, Batchuluun K, Ramadhani D, Syaidah R, Tsukada T, Azuma M, Kikuchi M, Yashiro T
  • Journal Title: Cell Tissue Res Volume: 359 Issue: 3 Pages: 909-914
  • DOI: 10.1007/s00441-014-2073-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Changes in fine structure of pericytes and novel desmin-immunopositive perivascular cells during postnatal development in rat anterior pituitary gland (2013)
  • Author(s): Jindatip D, Fujiwara K, Horiguchi K, Tsukada T, Kouki T, and Yashiro T
  • Journal Title: Anat Sci Int Volume: Vol. 88 Issue: 4 Pages: 196-203
  • DOI: 10.1007/s12565-013-0180-3
  • NAID: 10031194446
  • Peer Reviewed
• [Journal Article] Laminin and collagen modulate expression of the small leucine-rich proteoglycan fibromodulin in rat anterior pituitary gland (2013)
  • Author(s): Syaidah R, Horiguchi K, Fujiwara K, Tsukada T, Kikuchi M, and Yashiro T
  • Journal Title: Cell Tiss Res Volume: Vol. 354 Issue: 2 Pages: 633-638
  • DOI: 10.1007/s00441-013-1698-3
  • Peer Reviewed
• [Journal Article] Reassembly of Anterior Pituitary Organization by Hanging Drop Three-Dimensional Cell Culture (2013)
  • Author(s): Tsukada T, Kouki T, Fujiwara K, Ramadhani D, Horiguchi K, Kikuchi M, and Yashiro T
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 46 Issue: 4 Pages: 121-127
  • DOI: 10.1267/ahc.13015
  • NAID: 130003361079
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed
• [Presentation] Transforming growth factor beta 2 from folliculostellate cells induces collagen synthesis in pericytes of rat anterior pituitary gland (2015)
  • Author(s): Tsukada T, Ramadhani D, Syaidah R, Yashiro T
  • Organizer: 第120回日本解剖学会
  • Place of Presentation: 兵庫
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Identification of tissue inhibitors of metalloproteinases-expressing cells in rat anterior pituitary gland (2014)
  • Author(s): Azuma M, Batchuluun K, FujiwaraK, Tsukada T, Kikuchi M, Yashiro T
  • Organizer: 第39回日本比較内分泌学学会
  • Place of Presentation: 愛知
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] Immunohistochemical analysis of laminin isoform expression in diethylstilbestrol (DES)-treated rat as prolactinoma model (2014)
  • Author(s): Ramadhani D, Tsukada T, Syaidah R, Yashiro T
  • Organizer: 第18回日本内分泌病理学会
  • Place of Presentation: 東京
  • Year and Date: 2014-11-01 – 2014-11-02
• [Presentation] 下垂体前葉内S100タンパク質陽性細胞から分泌されるケモカインCXCL10のIFN-γによる制御 (2014)
  • Author(s): 堀口幸太郎、吉田彩舟、藤原研、樋口雅司、塚田岳大、加藤たか子、舘野こずえ、長谷川瑠美、瀧上 周、大迫俊二、屋代 隆、加藤幸雄
  • Organizer: 第41回日本神経内分泌学会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] ラット下垂体前葉におけるTGFb2の作用とTGFb受容体の組織内局在 (2014)
  • Author(s): 塚田岳大、藤原研、屋代隆
  • Organizer: 第55回日本組織細胞化学会
  • Place of Presentation: 長野
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] Alteration of laminin isoform expression in pituitary adenoma of diethylstilbestrol (DES)-treated rat (2014)
  • Author(s): Ramadhani D, Tsukada T, and Yashiro
  • Organizer: 第29回日本下垂体研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-08-08 – 2014-08-19
• [Presentation] 樹状細胞様S100タンパク質陽性細胞から分泌されるケモカインCXCL10の機能解析 (2014)
  • Author(s): 堀口幸太郎、吉田彩舟、藤原研、樋口雅司、塚田岳大、加藤たか子、舘野こずえ、長谷川瑠美、瀧上 周、大迫俊二、屋代 隆、加藤幸雄
  • Organizer: 第29回日本下垂体研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-08-08 – 2014-08-10
• [Presentation] ラット下垂体前葉における濾胞星状細胞と周皮細胞の相互作用： Transforming growth factor beta2を介したコラーゲン合成 (2014)
  • Author(s): 塚田岳大、Ramadhani Dini、藤原研、矢田部恵、喜幸富、屋代隆
  • Organizer: 第29回日本下垂体研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-08-08 – 2014-08-10
• [Presentation] Folliculostellate Cells Are Required for Laminin Release from Gonadotrophs in Rat Anterior Pituitary Gland (2014)
  • Author(s): Tsukada T, Azuma M, Ramadhani D, Batchuluun K, and Yashiro T
  • Organizer: 16th International Congress of Endocrinology and The Endocrine Society’s 96th Annual Meeting & Expo
  • Place of Presentation: Chicago, IL, USA
  • Year and Date: 2014-06-21 – 2014-06-24
• [Presentation] 下垂体前葉細胞のクロストーク―三次元培養法を用いた新たなアプローチ― (2014)
  • Author(s): 塚田岳大、屋代隆
  • Organizer: 第87回日本内分泌学会
  • Place of Presentation: 福岡
  • Year and Date: 2014-04-24 – 2014-04-26
  • Invited
• [Presentation] ラット下垂体前葉におけるエンケファリン前駆体遺伝子発現細胞の同定 (2014)
  • Author(s): 藤原研、塚田岳大、Tofrizal bin Alimuddin、Rita Maliza、Khongorzul Batchuluun、菊地元史、屋代隆
  • Organizer: 第119回日本解剖学会
  • Place of Presentation: 栃木
• [Presentation] ラット下垂体前葉におけるTGFbeta2を介した濾胞星状細胞と周皮細胞の新規細胞間相互作用 (2014)
  • Author(s): 塚田岳大、藤原研、Ramadhani Dini、矢田部恵、屋代隆
  • Organizer: 第119回日本解剖学会
  • Place of Presentation: 栃木
• [Presentation] aminin isoform expression during the anterior pituitary development of rat (2013)
  • Author(s): Ramadhani D, Tsukada T, and Yashiro T
  • Organizer: The Endocrine Society’s 95th Annual Meeting & Expo
  • Place of Presentation: San Francisco
• [Presentation] Folliculostellate cells interact with microvessel mural cell ‘pericyte’ to maintain collagen arrangement in rat anterior pituitary (2013)
  • Author(s): Tsukada T, Fujiwara K, Ramadhani D, Kouki T, Kikuchi M, and Yashiro T
  • Organizer: The Endocrine Society’s 95th Annual Meeting & Expo
  • Place of Presentation: San Francisco
• [Presentation] 胎生ラット下垂体におけるミッドカインの発現 (2013)
  • Author(s): 藤原研、Rita Maliza、Khongorzul Batchuluun、Dini Ramadhani、矢田部恵、塚田岳大、屋代隆
  • Organizer: 第28回日本下垂体研究会
  • Place of Presentation: 岩手
• [Presentation] Spatio-temporal expression of laminin isoforms in rat anterior pituitary gland during the postnatal development (2013)
  • Author(s): Ramadhani D, Tsukada T, Azuma M, Tofrizal A, Maliza R, Batchuluun K, Syaidah R, and Yashiro T
  • Organizer: 第17回日本内分泌病理学会
  • Place of Presentation: 神奈川
• [Presentation] ラット下垂体前葉におけるTGFb2の発現とその作用 (2013)
  • Author(s): 塚田岳大、堀口幸太郎、菊地元史、屋代隆
  • Organizer: 第38回日本比較内分泌学学会
  • Place of Presentation: 宮崎
• [Book] “B-type natriuretic peptide”, “C-type natriuretic peptides” and “Ventricular natriuretic peptide” in Handbook of Hormones: Comparative and General Endocrinology (2015)
  • Author(s): Tsukada T
  • Publisher: Elsevier
• [Book] "Evolution of Natriuretic Peptide System" in Reference Module in Biomedical Research (2014)
  • Author(s): Takei Y and Tsukada T
  • Total Pages: 6
  • Publisher: Elsevier
• [Book] Eel Physiology (2013)
  • Author(s): Tsukada T, Wong MK, Ogoshi M, and Yuge S
  • Total Pages: 370
  • Publisher: CRC press