| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
To elucidate the correlation between circadian clock development and differentiation stage of cancer cells, the circadian clock in the several human cancer cell lines were investigated. The circadian clock rhythm of these human cancer cell lines was not detected. However, the clock gene set in these cell lines was expressed. Next, the model of cancer development cell lines by myc overexpression using mouse embryonic stem cells (ESCs) was established. The in vitro differentiated ES cells during the induction of myc showed no circadian rhythm oscillation. The global gene expression analysis by RNA-seq identified the gene network inhibiting clock formation.
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