Epigenetic regulation of trophoblast lineage development

• Project/Area Number: 25860259
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human genetics
• Research Institution: National Center for Child Health and Development
• Principal Investigator: TOMIKAWA Junko 国立研究開発法人国立成育医療研究センター, その他部局等, 研究員 (80534990)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 胎盤 / エピゲノム / クロマチン構造 / クロマチン高次構造 / 細胞分化 / 幹細胞 / 初期発生 / クロマチン

Outline of Final Research Achievements

Division of embryonic and extraembryonic (trophoblast) cell lineages is the first differentiation event during early development. We generated genome-wide maps of epigenetic information in embryonic stem (ES) and trophoblast stem (TS) cells. From combined analysis with formaldehyde-assisted isolation of regulatory elements (FAIRE) and chromosome conformation capture (3C) techniques, we succeeded identification of enhancers of the Tead4 gene that is required for specification of trophectoderm in pre-implantation mammalian embryos.

Research Products

• [Journal Article] Molecular and Epigenetic Mechanism Regulating Hypothalamic Kiss1 Gene Expression in Mammals. (2016)
  • Author(s): Uenoyama Y, Tomikawa J, Inoue N, Goto T, Minabe S, Ieda N, Nakamura S, Watanabe Y, Ikegami K, Matsuda F, Ohkura S, Maeda KI, Tsukamura H.
  • Journal Title: Neuroendocrinology Volume: 印刷中 Issue: 6 Pages: 640-649
  • DOI: 10.1159/000445207
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of hypothalamic arcuate nucleus-specific enhancer region of kiss1 gene in mice. (2015)
  • Author(s): Goto, T., Tomikawa, J., Ikegami, K., Minabe, S., Abe, H., Fukanuma, T., Imamura, T., Takase, K., Sanbo, M., Tomita, K., Hirabayashi, M., Maeda, K., Tsukamura, H., Uenoyama, Y.
  • Journal Title: Mol Endocrinol Volume: 29 Issue: 1 Pages: 121-129
  • DOI: 10.1210/me.2014-1289
  • Peer Reviewed / Open Access
• [Journal Article] The role of maternal-specific H3K9me3 modification in establishing imprinted X-chromosome inactivation and embryogenesis in mice. (2014)
  • Author(s): Fukuda A, Tomikawa J, Miura T, Hata K, Nakabayashi K, Eggan K, Akutsu H, Umezawa A.
  • Journal Title: Nat Commun. Volume: 5 Issue: 1 Pages: 5464-5464
  • DOI: 10.1038/ncomms6464
  • Peer Reviewed / Open Access
• [Journal Article] <i>KISS1</i> Gene Expression in the Developing Brain of Female Pigs in Pre- and Peripubertal Periods (2014)
  • Author(s): Ieda, N., Uenoyama, Y., Tajima, Y., Nakata, T., Kano, M., Naniwa, Y., Watanabe, Y., Minabe, S., Tomikawa, J., Inoue, N., Matsuda, F., Ohkura, S., Maeda, K.-I. and Tsukamura, H.
  • Journal Title: Journal of Reproduction and Development Volume: 60 Issue: 4 Pages: 312-316
  • DOI: 10.1262/jrd.2013-129
  • NAID: 130004137827
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Presentation] Exploring the cell line-specific enhancers based on chromatin conformation (2015)
  • Author(s): Junko Tomikawa, Kohji Okamura, Keiko Hayashi, Hidenori Akutsu, Satoshi Tanaka, Kenichiro Hata, Kazuhiko Nakabayashi
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] マウス初期発生過程における細胞種特異的なクロマチン高次構造の解析 (2014)
  • Author(s): 冨川順子、岡村浩司、林恵子、阿久津英憲、田中智、秦健一郎、中林一彦
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Hi-C法による細胞種特異的なクロマチン高次構造解析 (2014)
  • Author(s): 冨川順子、前原一満、岡村浩司、林恵子、阿久津英憲、田中智、大川恭行、 秦健一郎、中林一彦
  • Organizer: 第８回エピジェネティクス研究会年会
  • Place of Presentation: 東京大学 伊藤国際学術研究センター
  • Year and Date: 2014-05-26 – 2014-05-27
• [Book] Astellas foundation for Research on Metabolic Disorders (2013)
  • Author(s): 冨川順子
  • Total Pages: 55
  • Publisher: 公益財団法人アステラス病態代謝研究会