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Analysis of IL-10 produced by Bordetella effector BopN.

Research Project

Project/Area Number 25860324
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bacteriology (including mycology)
Research InstitutionKanagawa Academy of Science and Technology

Principal Investigator

SAYURI Chiba  公益財団法人神奈川科学技術アカデミー, 光触媒グループ抗菌・抗ウイルス研究グループ, 研究員 (90528150)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords感染免疫 / 病原因子 / 細菌感染
Outline of Final Research Achievements

One of Bordetella effector BopN is translocated itself into the nucleus and suppresses a host immune response by enhanced production of IL-10. BopN also induces a nuclear translocation of NF-κB p50, but inhibits of NF-κB p65. However, the molecular details of BopN function are unknown. To investigate the mechanism of the BopN nuclear import, coprecipitation analysis was carried out. It was found that BopN associates with importin β1, a nuclear import factor. Next, the nuclear import of BopN was analyzed using expression vectors producing full length or various truncated BopN. The nuclear import of BopN was detected in all derivatives except 81-100 a.a deletion in BopN, and translocated BopN into nucleus was colocalized with importin β1. Moreover, the nuclear translocation of NF-κB p65 was not inhibited in 61-120 a.a deletion in BopN. Taken together, BopN 81-100 a.a. is involved in the importin β1-mediated nuclear transport and may affect the subsequent signal transductions.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] Aryl hydrocarbon receptor protects against bacterial infection by promoting macrophage survival and reactive oxygen species production2014

    • Author(s)
      Kimura A, Abe H, Tsuruta S, Chiba S, Fujii-Kuriyama Y, Sekiya T, Morita R, Yoshimura A.
    • Journal Title

      Int Immunol.

      Volume: 26 Issue: 4 Pages: 209-220

    • DOI

      10.1093/intimm/dxt067

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 加齢による免疫低下と高齢者敗血症2013

    • Author(s)
      井上茂亮, 千葉紗由利, 小森由香子, 猪口貞樹
    • Journal Title

      ICUとCCM

      Volume: 37(8) Pages: 577-584

    • Related Report
      2013 Research-status Report
  • [Presentation] Immunosenescence and sepsis in the elderly.2013

    • Author(s)
      井上茂亮、千葉紗由利、小森由香子、猪口貞樹
    • Organizer
      第26回日本外科感染症学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] Inactivation of virus by photocatalysis.

    • Author(s)
      原正幸、千葉紗由利、石黒斉、中野竜一、三木慎一郎、窪田吉信
    • Organizer
      第20回記念シンポジウム「光触媒の最近の展開」
    • Place of Presentation
      東京大学生産技術研究所コンベンションホール
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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