The analysis for maintenance of function and chemokine receptor expression in human cytotoxic T lymphocytes under exposure to asbestos

• Project/Area Number: 25860470
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hygiene and public health
• Research Institution: Kawasaki Medical School
• Principal Investigator: TAKEI NAOKO 川崎医科大学, 医学部, 助教 (00509276)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 石綿 / 中皮腫 / CTL / ケモカイン受容体 / granzyme B / perforin / ケモカイン

Outline of Final Research Achievements

Chrysotile B (CB) asbestos exposure suppressed the induction of CTL during MLR, accompanied by the partial decrease in %CXCR3+ cells in CD8+T cells. We also prepared a sub-line of human CD8+T cells exposed to 5 μg/ml CB and analyzed the effect of CB exposure on CTL. Granzyme B+, perforin+, and IFN-gamma+ cells did not decrease in a sub-line of CD8+T cells exposed CB.

Research Products

• [Journal Article] Functional alteration of natural killer cells and cytotoxic T lymphocytes upon asbestos exposure and in malignant mesothelioma patients. (2015)
  • Author(s): Nishimura, Y., Kumagai-Takei, N., Matsuzaki, H., Lee, S., Maeda, M., Kishimoto, T., Fukuoka, K., Nakano, T., Otsuki, T.
  • Journal Title: Biomed. Res. Int. Volume: 2015 Pages: 1-9
  • DOI: 10.1155/2015/238431
  • Peer Reviewed / Open Access
• [Journal Article] Functional properties of CD8+ lymphocytes in patients with pleural plaque and malignant mesothelioma (2014)
  • Author(s): Kumagai-Takei N, Nishimura Y, Maeda M, Hayashi H, Matsuzaki H, Lee S, Kishimoto T, Fukuoka K, Nakano T, and Otsuki T
  • Journal Title: J Immunol Res Volume: 2014 Pages: 10-20
  • DOI: 10.1155/2014/670140
  • Peer Reviewed / Open Access
• [Presentation] Effect Of IL-2 Addition on Granzyme B Expression and Proliferation in CD8+ Lymphocytes Exposed to Asbestos During MLR (2016)
  • Author(s): Kumagai-Takei N. Nishimura Y, Matsuzaki H, Lee S, Yoshitome K, Otsuki T.
  • Organizer: The 55th Annual Meeting of the Society of Toxicology
  • Place of Presentation: Ernest N. Morial Convention Center (New Orleans, LA)
  • Year and Date: 2016-03-13
  • Int'l Joint Research
• [Presentation] Induction of granzyme B expression in CD8+ lymphocytes exposed to asbestos during MLR by IL-2 addition (2015)
  • Author(s): Kumagai-Takei N, Nishimura Y, Matsuzaki H, Lee S, Otsuki T.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター (札幌市）
  • Year and Date: 2015-11-18
• [Presentation] CTL 分化・機能に及ぼす石綿曝露影響の機序解析 (2015)
  • Author(s): 武井直子，西村泰光，松﨑秀紀，李順姫，吉留敬，林宏明，大槻剛巳．
  • Organizer: 第22回日本免疫毒性学会学術年会
  • Place of Presentation: 京都大学百周年時計台記念館国際交流ホール (京都市)
  • Year and Date: 2015-09-10
• [Presentation] Functional properties of CD8+ lymphocytes in asbestos-exposed patients with pleural plaque and malignant mesothelioma (2015)
  • Author(s): Kumagai-Takei N, Nishimura Y, Maeda M, Hayashi H, Matsuzaki H, Lee S, Kishimoto T, Fukuoka K, Nakano T, Otsuki T.
  • Organizer: 31st International Congress on Occupational Health
  • Place of Presentation: COEX Convention Center (Seoul, Korea)
  • Year and Date: 2015-05-31
  • Int'l Joint Research
• [Presentation] Effect of IL-2 addition on the suppressed induction of human CTL in mixed lymphocyte reactions upon exposure to asbestos (2015)
  • Author(s): Kumagai-Takei N, Nishimura Y, Matsuzaki H, Lee S, Hayashi H, Otsuki T.
  • Organizer: 31st International Congress on Occupational Health
  • Place of Presentation: COEX Convention Center (Seoul, Korea)
  • Year and Date: 2015-05-31
  • Int'l Joint Research
• [Presentation] CTL分化誘導時の石綿曝露によるCD8+細胞の細胞傷害性低下に及ぼすIL-2の影響 (2015)
  • Author(s): 武井直子，西村泰光，松崎秀紀，李順姫，吉留敬，林宏明，大槻剛巳．
  • Organizer: 第85回日本衛生学会学術総会
  • Place of Presentation: 和歌山県民文化会館・ホテルアバローム紀の国（和歌山市）
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 石綿曝露によるnaive CD8+T細胞からCTLへの分化抑制におけるIL-2関与の検討 (2015)
  • Author(s): 武井直子，西村泰光，松﨑秀紀，李順姫，吉留敬，林宏明，大槻剛巳．
  • Organizer: 第14回 分子予防環境医学研究会
  • Place of Presentation: 大阪市立大学（大阪市）
  • Year and Date: 2015-02-13 – 2015-02-14
• [Presentation] ケモカインレセプターに注目したエフェクター/メモリーCTL機能に関する石綿関連る石綿関連分子指標の探索
  • Author(s): 武井 直子
  • Organizer: 第4回 川崎医科大学学術集会
  • Place of Presentation: 川崎医科大学（岡山県倉敷市）
• [Book] Biological Effects of Fibrous and Particulate Substances, (Series Title: Current Topics in Environmental Health and Preventive Medicine) (2015)
  • Author(s): Kumagai-Takei N, Nishimura Y, Matsuzaki H, Maeda M, Lee S, Yoshitome K, Otsuki T.
  • Total Pages: 221
  • Publisher: Springer Tokyo