| Project/Area Number |
25860516
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
KASHIMA Shin 旭川医科大学, 医学部, 助教 (10548655)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | プロバイオティクス / 腸管炎症 / 腸管線維化 / ポリリン酸 / 腸内細菌由来活性物質 |
|---|
| Outline of Final Research Achievements |
This study investigated whether polyphosphate (poly P), an active molecule derived from Lactobacillus brevis, could improve the inflammation and fibrosis in DSS-induced and TNBS-induced colitis models. The histological inflammation and fibrosis were improved, and the levels of IL-1β and TNFα as well as TGF-β1 and collagens were decreased in the group administered poly P. To clarify the mechanism responsible for the observed effects, Caco-2/BBE and THP-1 cells were treated with LPS to induce inflammation. CCD-18 cells were treated with TGF-β1 to induce fibrosis. The expressions of IL-1β and TGFβ1 in Caco-2/BBE cells and of TNFα in THP-1 cells were reduced by poly P. Poly P did not affect the expression of collagens and CTGF in CCD-18 cells. These results indicate that poly P regulates intestinal inflammation and fibrosis by down-regulating the expression of inflammation- and fibrosis-associated mediators in the intestinal epithelia and immune cells.
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