The therapeutic effects of novel probiotics-derived molecules on the intestinal inflammation and fibrosis in chronic colitis models
Project/Area Number |
25860516
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
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Research Institution | Asahikawa Medical College |
Principal Investigator |
KASHIMA Shin 旭川医科大学, 医学部, 助教 (10548655)
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Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | プロバイオティクス / 腸管炎症 / 腸管線維化 / ポリリン酸 / 腸内細菌由来活性物質 |
Outline of Final Research Achievements |
This study investigated whether polyphosphate (poly P), an active molecule derived from Lactobacillus brevis, could improve the inflammation and fibrosis in DSS-induced and TNBS-induced colitis models. The histological inflammation and fibrosis were improved, and the levels of IL-1β and TNFα as well as TGF-β1 and collagens were decreased in the group administered poly P. To clarify the mechanism responsible for the observed effects, Caco-2/BBE and THP-1 cells were treated with LPS to induce inflammation. CCD-18 cells were treated with TGF-β1 to induce fibrosis. The expressions of IL-1β and TGFβ1 in Caco-2/BBE cells and of TNFα in THP-1 cells were reduced by poly P. Poly P did not affect the expression of collagens and CTGF in CCD-18 cells. These results indicate that poly P regulates intestinal inflammation and fibrosis by down-regulating the expression of inflammation- and fibrosis-associated mediators in the intestinal epithelia and immune cells.
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Report
(3 results)
Research Products
(1 results)
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[Journal Article] Polyphosphate, an active molecule derived from probiotic Lactobacillus brevis, improves the fibrosis in murine colitis.2015
Author(s)
Kashima S, Fujiya M, Konishi H, Ueno N, Inaba Y, Moriichi K, Tanabe H, Ikuta K, Ohtake T, Kohgo Y.
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Journal Title
Transl Res.
Volume: in press
Issue: 2
Pages: 00069-9
DOI
NAID
Related Report
Peer Reviewed / Open Access