| Project/Area Number |
25860542
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Kyushu University |
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Principal Investigator |
Arita Shuji 九州大学, 医学(系)研究科(研究院), 助教 (20544935)
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| Co-Investigator(Renkei-kenkyūsha) |
ARIYAMA Hiroshi 九州大学病院, 血液・腫瘍内科, 助教 (80713437)
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| Research Collaborator |
NAKANO Michitaka
TANAKA Mamoru
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 大腸がん / 単細胞解析 / がん幹細胞 / TGF-β / TGFβ / がん性腹水 / 腹腔内マクロファージ / がんの単細胞遺伝子解析 / 消化管がん / 末梢血循環がん細胞 |
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| Outline of Final Research Achievements |
This research program was based on a concept to analyze intratumor heterogeneity of gastrointestinal cancers by single cell-based genetic assay. Although originally planned to analyze intratumor clonal transision and drug resistance of gastric cancer, it was difficult to establish an assay of human gastric cancer. We consequently conducted experiments on human colorectal cancer to analyze cancer stemness and intratumor clonality. We achieved an experimental system of organoid formation from patient-derived CD44-positive colorectal cancer cells. Furthermore, CD44-negative cells also formed organoids under a condition of epidermal-mesenchimal transision. TWIST1 and TGF-beta signal pathway were involved in this phenomenon.
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