Analysis of drug sensitivity and resistance by single cell-based genetic assay and intratumoral profiling of gastric cancer
Project/Area Number |
25860542
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
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Research Institution | Kyushu University |
Principal Investigator |
Arita Shuji 九州大学, 医学(系)研究科(研究院), 助教 (20544935)
|
Co-Investigator(Renkei-kenkyūsha) |
ARIYAMA Hiroshi 九州大学病院, 血液・腫瘍内科, 助教 (80713437)
|
Research Collaborator |
NAKANO Michitaka
TANAKA Mamoru
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 大腸がん / 単細胞解析 / がん幹細胞 / TGF-β / TGFβ / がん性腹水 / 腹腔内マクロファージ / がんの単細胞遺伝子解析 / 消化管がん / 末梢血循環がん細胞 |
Outline of Final Research Achievements |
This research program was based on a concept to analyze intratumor heterogeneity of gastrointestinal cancers by single cell-based genetic assay. Although originally planned to analyze intratumor clonal transision and drug resistance of gastric cancer, it was difficult to establish an assay of human gastric cancer. We consequently conducted experiments on human colorectal cancer to analyze cancer stemness and intratumor clonality. We achieved an experimental system of organoid formation from patient-derived CD44-positive colorectal cancer cells. Furthermore, CD44-negative cells also formed organoids under a condition of epidermal-mesenchimal transision. TWIST1 and TGF-beta signal pathway were involved in this phenomenon.
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Report
(4 results)
Research Products
(12 results)
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[Presentation] TGF-beta signaling pathway induced cancer stem-like properties in human CD44- colorectal cancer cells2016
Author(s)
Michitaka Nakano, Mamoru Tanaka, Taichi Isobe, Yasufumi Uehara, Kohta Miyawaki, Yoshikane Kikushige, Shinichi Mizuno, Kenji Tsuchihashi, Shuji Arita, Hiroshi Ariyama, Hitoshi Kusaba, Takashi Ueki, Koichi Akashi and Eishi Baba
Organizer
AACR annual meeting 2016
Place of Presentation
New Orleans
Year and Date
2016-04-16
Related Report
Int'l Joint Research
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[Presentation] 上皮転換によるがん幹細胞性の獲得2016
Author(s)
Michitaka Nakano, Mamoru Tanaka, Taichi Isobe, Kohta Miyawaki, Yoshikane Kikushige, Shinichi Mizuno, Shigeo Takaishi, Hitoshi Kusaba, Takashi Ueki, Eishi Baba, Koichi Akashi
Organizer
新学術領域「がん支援」
Place of Presentation
大津
Year and Date
2016-02-03
Related Report
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