Development of the method for predicting sensitivity to chemoradiothrapy for esophageal cancer using comprehensive gene expression profiling analysis

• Project/Area Number: 25860546
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Sapporo Medical University
• Principal Investigator: OHNUMA Hiroyuki 札幌医科大学, 医学部, 助教 (60582997)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 食道癌 / 化学放射線療法 / 感受性予測 / miRNA / 効果予測因子

Outline of Final Research Achievements

We performed comprehensive miRNA expression profiling analysis in pretherapeutic biopsies of responders and nonresponders in chemoradiotherapy using docectacel, nedaplatin and 5-FU of esophageal cancer, and the miRNA profiling revealed the expression of hsa-miR-625-3p and hsa-miR-4731-3p was significantly higher in the responder group. As validation analysis, we are examining the mechanism for acquiring CRT resistance using esophageal cancer cell lines transfected with the candidate miRNAs.

Research Products

• [Presentation] 進行食道癌に対するdocetaxel，nedaplatin, 5-FU，放射線同時併用療法（DNF-R）の第Ⅰ/Ⅱ相試験 (2015)
  • Author(s): 大沼啓之，佐藤康史，大須賀崇裕，佐川保，林毅，佐藤勉，宮西浩嗣，小船雅義，瀧本理修，堀正和，染谷正則，中田健生，坂田耕一，加藤淳二
  • Organizer: 第42回北海道食道癌研究会
  • Place of Presentation: 札幌市教育文化会館 (北海道，札幌市)
  • Year and Date: 2015-04-11
• [Presentation] A phase I/II study of definitive chemoradiotherapy with docetaxel, nedaplatin, and 5-fluorouracil (DNF-R) for advanced esophageal cancer. (2015)
  • Author(s): Yasushi Sato, Hiroyuki Ohnuma, Masahiro Hirakawa, Yutaka Okagawa, Takahiro Osuga, Kazuyuki Murase, Kohichi Takada, Yutaka Kawano, Satoshi Iyama, Tsuyoshi Hayashi, Tsutomu Sato, Koji Miyanishi, Rishu Takimoto, Masayoshi Kobune, Tamotsu Sagawa, Masakazu Hori, Masanori Someya, Koh-ichi Sakata, Tetsuji Takayama, Junji Kato
  • Organizer: 2015 Gastrointestinal Cancers Symposium of the American Society of Clinical Oncology
  • Place of Presentation: Moscone West Building (San Francisco, CA)
  • Year and Date: 2015-01-15 – 2015-01-17
• [Presentation] 進行食道癌に対するDocetaxel/ CDGP/ 5-FU・放射線同時併用療法による第I/II相試験 -中間報告-
  • Author(s): 佐藤康史，他
  • Organizer: 第40回北海道食道癌研究会
  • Place of Presentation: 札幌市