Loss of cell polarity and the role of autophagy in entosis

• Project/Area Number: 25860561
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Juntendo University
• Principal Investigator: INAMI YOSHIHIRO 順天堂大学, 医学部, 助教 (70445500)
• Research Collaborator: YAMASHINA Shunhei 順天堂大学, 医学部, 准教授 (30338412)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: エントーシス / 肝癌 / オートファジー / 細胞極性 / 癌細胞 / 肝癌細胞

Outline of Final Research Achievements

The recognition of the tumor abiogenesis cells due to the neighboring normal cells equalled the tumor cells which lost cellular polarity and evaluated entosis which was an exclusion mechanism with in vivo and in vitro.
We made hepatocellular carcinoma which made p62 suffer a loss and observed it whether you occurred entosis using hepatocellular carcinoma. p62 deficiency hepatocellular carcinoma phagocyted hepatocellular carcinoma, culture day 2, and it was observed that we migrated it so that p62 deficiency cells surrounded it and inhibited division, growth of hepatocellular carcinoma.