Role of Hrs in life cycle of hepatitis C virus

• Project/Area Number: 25860572
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Miyagi Prefectural Hospital Organization Miyagi Cancer Center
• Principal Investigator: TAMAI Keiichi 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), その他部局等, 研究員 (40509262)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: HCV / Hrs / ESCRT / 小胞輸送

Outline of Final Research Achievements

To evaluate the role of Hrs, known as ESCRT proteins, in HCV life cycle, we check the HCV-RNA in serum of chimeric liver mice. We demonstrate that the decrease of HCV-RNA in mouse serum when hrs is knocked down using adenoviral shRNA vector. Further study will be needed to elucidate the precise mechanisms of hrs-dependent HCV life cycle in humanized mice liver.

Research Products

• [Journal Article] Suppressive expression of CD274 increases tumorigenesis and cancer stem cell phenotypes in cholangiocarcinoma (2014)
  • Author(s): Keiichi Tamai, Mao Nakamura, Masamichi Mizuma, Mai Mochizuki, Misa Yokoyama, Hiroyuki Endo, Kazunori Yamaguchi, Takayuki Nakagawa, Masaaki Shiina, Michiaki Unno, Koji Muramoto, Ikuro Sato, Kennichi Satoh, Kazuo Sugamura, and Nobuyuki Tanaka
  • Journal Title: Cancer Science Volume: 105 Issue: 6 Pages: 667-674
  • DOI: 10.1111/cas.12406
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer (2014)
  • Author(s): Nakagawa T, Endo H, Yokoyama M, Abe J, Tamai K, Tanaka N, Sato I, Takahashi S, Kondo T, Satoh K.
  • Journal Title: Biochem Biophy Res Commun Volume: 436 Issue: 2 Pages: 319-324
  • DOI: 10.1016/j.bbrc.2013.05.101
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] CD271 Defines a Stem Cell-Like Population in Hypopharyngeal Cancer (2013)
  • Author(s): Imai T
  • Journal Title: PLoS One Volume: 8 Issue: 4 Pages: e62002-e62002
  • DOI: 10.1371/journal.pone.0062002
  • Peer Reviewed
• [Journal Article] Enhanced expression of long non-coding RNA HOTAIR is associated with the development of gastric cancer. (2013)
  • Author(s): Endo H, Shiroki T, Nakagawa T, Yokoyama M, Tamai K, Yamanami H, Fujiya T, Sato I, Yamaguchi K, Tanaka N, Iijima K, Shimosegawa T, Sugamura K, Satoh K.
  • Journal Title: PlosONE Volume: 8 Issue: 10 Pages: e77070-e77070
  • DOI: 10.1371/journal.pone.0077070
  • Peer Reviewed