Multilayered cell sheets created by a novel magnetite tissue engineering method for myocardial infarction

• Project/Area Number: 25860595
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Kagoshima University (2014); Nagoya University (2013)
• Principal Investigator: ISHII Masakazu 鹿児島大学, 医歯(薬)学総合研究科, 助教 (00456683)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 再生医療 / 脂肪由来幹細胞 / 細胞シート / 心筋梗塞 / 血管新生

Outline of Final Research Achievements

Most common method of stem/progenitor cell transplantation is direct myocardium injections of cell suspensions using a syringe and needle. However, some reports showed that this needle injection method has several disadvantages, including cell loss caused by leakage of injected cells. To overcome these problems, several tissue engineering (TE) technologies are emerging for regenerative medicine.
ADRC sheets were successfully engrafted into ischemic heart. Transplantation of ADRC sheets, created by the Mag-TE system and the ECM, improved infarct wall thining and significantly inhibited fibrosis than control group. ADRC sheets transplantation significantly improved cardiac function, and increased VEGF and bFGF mRNA expression in ischemic hearts.Implantation of ADRC sheets protects the heart against pathological cardiac remodeling through its ability to promote angiogenesis. ADRC sheets may become a novel regenerative medicine strategy for ischemic heart disease in future.

Research Products

• [Journal Article] IRAP deficiency attenuates diet-induced obesity in mice through increased energy expenditure. (2015)
  • Author(s): Niwa M, Numaguchi Y, Ishii M, Kuwahata T, Kondo M, Shibata R, Miyata K, Oike Y, Murohara T.
  • Journal Title: Biochem Biophys Res Commun. Volume: 457(1) Issue: 1 Pages: 12-8
  • DOI: 10.1016/j.bbrc.2014.12.071
  • Peer Reviewed
• [Journal Article] Multilayered adipose-derived regenerative cell sheets created by a novel magnetic tissue engineering method for myocardial infarction. (2014)
  • Author(s): Ishii M, Shibata R, Shimizu Y, Yamamoto T, Kondo K, Inoue Y, Ouchi N, Tanigawa T, Kanemura N, Ito A, Honda H, Murohara T.
  • Journal Title: Int J Cardiol. Volume: 175 Issue: 3 Pages: 545-553
  • DOI: 10.1016/j.ijcard.2014.06.034
  • Peer Reviewed / Open Access
• [Journal Article] Vildagliptin stimulates endothelial cell network formation and ischemia-induced revascularization via an endothelial nitric oxide synthase-dependent mechanism (2014)
  • Author(s): Ishii M, Shibata R, Kondo K, Kambara T, Shimizu Y, Tanigawa T, Bando YK, Nishimura M, Ouchi N, Murohara T.
  • Journal Title: J Biol Chem Volume: 289 Issue: 39 Pages: 27235-45
  • DOI: 10.1074/jbc.m114.557835
  • Peer Reviewed / Open Access
• [Journal Article] Therapeutic Angiogenesis by Autologous Adipose-Derived Regenerative Cells: Comparison With Bone Marrow Mononuclear Cells. (2014)
  • Author(s): 31.Hao CN, Shintani S, Shimizu Y, Kondo K, Ishii M, Wu H, Murohara T.
  • Journal Title: Am J Physiol Heart Circ Physiol. Volume: 307 Issue: 6 Pages: H869-H879
  • DOI: 10.1152/ajpheart.00310.2014
  • Peer Reviewed
• [Journal Article] Adiponectin deficiency exacerbates age-related hearing impairment (2014)
  • Author(s): Tanigawa T, Shibata R, Ouchi N, Kondo K, Ishii M, Katahira N, Kambara T, Inoue Y, Takahashi R, Ikeda N, Kihara S, Ueda H, Murohara T
  • Journal Title: Cell Death Dis Volume: 5 Issue: 4 Pages: e1189-e1189
  • DOI: 10.1038/cddis.2014.140
  • Peer Reviewed
• [Journal Article] Adiponectin-mediated modulation of lymphatic vessel formation and lymphedema (2013)
  • Author(s): Shimizu Y, Shibata R, Ishii M, Ohashi K, Kambara T, Uemura Y, Yuasa D, Kataoka Y, Kihara S, Murohara T, Ouchi N
  • Journal Title: J Am Heart Assoc Volume: 2 Issue: 5
  • DOI: 10.1161/jaha.113.000438
  • Peer Reviewed
• [Journal Article] Therapeutic Reendothelialization by Induced Pluripotent Stem Cells After Vascular Injury (2013)
  • Author(s): Yamamoto T, Shibata R, Ishii M, Kanemura N, Kito T, Suzuki H, Miyake H, Maeda K, Tanigawa T, Ouchi N, Murohara T
  • Journal Title: Arterioscler Thromb Vasc Biol Volume: 33 Issue: 9 Pages: 2218-21
  • DOI: 10.1161/atvbaha.113.301313
  • Peer Reviewed
• [Journal Article] iPS cell sheets created by a novel magnetite tissue engineering method for reparative angiogenesis. (2013)
  • Author(s): Kito T, Shibata R, Ishii M, Suzuki H, Himeno T, Kataoka Y, Yamamura Y, Yamamoto T, Nishio N, Ito S, Numaguchi Y, Tanigawa T, Yamashita JK, Ouchi N, Honda H, Isobe K, Murohara T.
  • Journal Title: Sci Rep Volume: 11:3 Issue: 1 Pages: 1418-1418
  • DOI: 10.1038/srep01418
  • Peer Reviewed