Research Project
Grant-in-Aid for Young Scientists (B)
In this study, I succeeded to generate cardiac-specific NRSF conditional knockout mice (NRSF ccKO) and found that these mice show cardiac dysfunction, malignant arrhythmias and sudden cardiac death, implying that NRSF plays an essential role in maintaining normal cardiac function. To address the underlying mechanisms, I crossed these mice with HDAC1/2 cardiac-specific double heterozygote knockout mice and analyzed NRSF/HDAC1/2 cardiac-specific triple heterozygote knockout mice. I also found that GNAO1 gene expression is increased in NRSF ccKO. To examine the contribution of G alpha O to cardiac dysfunction seen in NRSF ccKO, I treated these mice with PTX and found that PTX improved cardiac function and the survival curve in NRSF ccKO. Currently I am further analyzing the role of G alpha O in the phenotype of NRSF ccKO.
All 2015 2014 2013 Other
All Journal Article (6 results) (of which Peer Reviewed: 6 results, Open Access: 3 results) Presentation (3 results) Remarks (2 results)
Journal of Molecular and Cellular Cardiology
Volume: 80 Pages: 81-89
10.1016/j.yjmcc.2014.12.019
120007100131
Cardiovasc Research
Volume: 104 Issue: 1 Pages: 183-193
10.1093/cvr/cvu185
120005619790
PLoS One
Volume: 25 Issue: 3 Pages: e92314-e92314
10.1371/journal.pone.0092314
120007100132
British Journal of Pharmacology
Volume: 170(7) Issue: 7 Pages: 1384-1395
10.1111/bph.12328
Journal of the American Heart Association
Volume: 2(3) Issue: 3
10.1161/jaha.113.000150
PLoS ONE
Volume: 8(1) Issue: 1 Pages: e53233-e53233
10.1371/journal.pone.0053233
120005241437
http://www.kuhp.kyoto-u.ac.jp/~med2/jpn/research/cardio.html