Clarification of the mechanism how human cardiac progenitor cells differentiate into cardiomyocytes.

• Project/Area Number: 25860603
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Okayama University
• Principal Investigator: YOSHIDA Masashi 岡山大学, 医歯(薬)学総合研究科, 助教 (70532761)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 心臓内幹細胞 / 内皮細胞 / DNAマイクロアレイ

Outline of Final Research Achievements

We compared expressed genes in human cardiac progenitor cells before and after cardiac differentiation by DNA microarray. We differentiated human cardiac progenitor cells into cardiomyocytes by co-culture those cells with neonatal rat ventricular myocytes. This comparison revealed that a set of genes which promote cells into endothelial cells was expressed in human cardiac progenitor cells. But a set of genes which are known to promote embryonic stem cells into cardiomyocytes was not able to be compared because DNA microarray chips reacted with rat genes. Careful interpretation may be need to understand the mechanism how human cardiac progenitor cells differentiate into cardiomyocytes with these results. On the other hand, we found that human progenitor cells can be transdifferentiate into cardiomyocytes by co-culture those cells with human endothelial cells

Research Products

• [Journal Article] Directed Differentiation of Patient-Specific Induced Pluripotent Stem Cells Identifies the Transcriptional Repression and Epigenetic Modification of NKX2-5, HAND1, and NOTCH1 in Hypoplastic Left Heart Syndrome (2014)
  • Author(s): Junko Kobayashi, Masashi Yoshida, Suguru Tarui, Masataka Hirata, Yusuke Nagai, Shingo Kasahara, Kenji Naruse, Hiroshi Ito, Shunji Sano, Hidemasa Oh
  • Journal Title: PLOS one Volume: 9
  • NAID: 120005553911
  • Peer Reviewed
• [Journal Article] Effect of vildagliptin, a dipeptidyl peptidase 4 inhibitor, on cardiac hypertrophy induced by chronic beta-adrenergic stimulation in rats (2014)
  • Author(s): Miyoshi T, Nakamura K, Yoshida M, Miura D, Oe H, Akagi S, Sugiyama H, Akazawa K, Yonezawa T, Wada J, Ito H
  • Journal Title: Cardiovasc Diabetol Volume: 13 Issue: 1 Pages: 43-43
  • DOI: 10.1186/1475-2840-13-43
  • Peer Reviewed
• [Presentation] T-box 5 controls age-dependent cardiogenic activity in human cardiac progenitor cells through insulin-like growth factor-1 receptor signaling (2014)
  • Author(s): Masashi Yoshida, Suguru Tarui, Junko Kobayashi, Toru Miyoshi, Youhei Maeshima, Shunji Sano, Hiroshi Ito, Hidemasa Oh
  • Organizer: ESC2014
  • Place of Presentation: バルセロナ
  • Year and Date: 2014-08-30 – 2014-09-03