| Project/Area Number |
25860761
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
TAKAHASHI Akinori 沖縄科学技術大学院大学, その他の研究科, 研究員 (90637589)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Obesity / The CCR4-NOT complex / mRNA decay / 肥満 / mRNA分解 / CCR4-NOT複合体 / 翻訳 / mRNA 分解 / CCR4-NOT 複合体 / インスリンシグナル |
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| Outline of Final Research Achievements |
Obesity is a risk factor of various lifestyle diseases. Understanding of detail molecular mechanisms underlying obesity is required for invention of novel therapeutic approaches. The CCR4-NOT complex is a major eukaryotic deadenylase, and CNOT8 is one of the enzymatic subunits. The CCR4-NOT complex is implicated in obesity. To investigate molecular mechanism, we used liver-specific CNOT8 knockout mice model. We found that CNOT8 negatively regulates Egfr expression and contributes to obesity. Interestingly, CNOT8 regulates Egfr mRNA via deadenylation-independent post-transcriptional mechanism. Our study will provide a novel molecular mechanism of obesity and a new in vivo model of deadenylation-independent prost-transcriptional regulation.
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