| Project/Area Number |
25860764
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Endocrinology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
Takishima Shigeru 東京医科歯科大学, 医学部附属病院, 非常勤講師 (70648146)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 細胞分化 / 副腎髄質 / Δ5ステロイド / 副腎 / 発生 / 副腎皮質 / Δ５ステロイド |
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| Outline of Final Research Achievements |
For elucidating the role of delta-5 steroid for neurogenic cells, we used SK-N-BE cells as a model of human nurogenic cells. Although, Pregnenolone and 17-OH pregnenolone significantly activated the proliferation of the cells by 20%, DHEA strongly suppressed by 70%, suggesting that delta-5 steroids regulate the proliferation of neurogenic cells. In order to clarify the molecular mechanisms, we evaluated the change of the genes in SK-N-BE cells by delta-5 steroids. DHEA increased the expression of Nestin twice. Nestin is a filament protein and specifically expressed in neurogenic ectoderm cells, and recently it has been suggested to have a role for signal transduction into nucleus and for cell metabolism. We speculate that delta 5 steroids regulate the differentiation and the proliferation of neurogenic cells via those mechanisms.
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