Transient Tcf3 Gene Repression by TALE-Transcription Factor Targeting
| Project/Area Number |
25860797
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Okayama University |
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Principal Investigator |
Masuda Junko 岡山大学, 自然科学研究科, 助教 (20424674)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | TCF3 (E2A) / 人工転写因子 / TALE / TALE技術 / 造血幹細胞 / E2A / 体性幹細胞 / 免疫細胞療法 |
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| Outline of Final Research Achievements |
Transplantation of hematopoietic stem and progenitor cells (HSCs) is now a widely performed therapy for many hematopoietic diseases. However, these cells are present in low number and are subject to replicative senescence after extraction; thus, the acquisition of sufficient numbers of cells for transplantation requires donors able to provide repetitive blood samples and/or methods of expanding cell numbers without disturbing cell multipotentiality. Previous studies have shown that HSCs maintain their multipotentiality and self-renewal activity if TCF3 transcription function is blocked under B cell differentiating conditions. Taking advantage of this finding to devise a new approach to HSC expansion in vitro, we constructed an episomal expression vector that specifically targets and transiently represses the TCF3 gene.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Evaluation of glycosylated docetaxel-encapsulated liposomes prepared by remote loading under solubility gradient.2016
Author(s)
Tsukasa Shigehiro, Wenjia Zhai, Arun Vaidyanath, Junko Masuda, Akifumi Mizutani, Tomonari Kasai, Hiroshi Murakami, Hiroki Hamada, David S Salomon, Katsuhiko Mikuni, Yuhki Seno, Tadakatsu Mandai, Masaharu Seno
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Journal Title
Journal of Microencapsulation
Volume: 33
Issue: 2
Pages: 172-182
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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