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Transient Tcf3 Gene Repression by TALE-Transcription Factor Targeting

Research Project

Project/Area Number 25860797
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Hematology
Research InstitutionOkayama University

Principal Investigator

Masuda Junko  岡山大学, 自然科学研究科, 助教 (20424674)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsTCF3 (E2A) / 人工転写因子 / TALE / TALE技術 / 造血幹細胞 / E2A / 体性幹細胞 / 免疫細胞療法
Outline of Final Research Achievements

Transplantation of hematopoietic stem and progenitor cells (HSCs) is now a widely performed therapy for many hematopoietic diseases. However, these cells are present in low number and are subject to replicative senescence after extraction; thus, the acquisition of sufficient numbers of cells for transplantation requires donors able to provide repetitive blood samples and/or methods of expanding cell numbers without disturbing cell multipotentiality. Previous studies have shown that HSCs maintain their multipotentiality and self-renewal activity if TCF3 transcription function is blocked under B cell differentiating conditions. Taking advantage of this finding to devise a new approach to HSC expansion in vitro, we constructed an episomal expression vector that specifically targets and transiently represses the TCF3 gene.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (18 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 1 results) Book (2 results)

  • [Int'l Joint Research] 国立衛生研究所(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Evaluation of glycosylated docetaxel-encapsulated liposomes prepared by remote loading under solubility gradient.2016

    • Author(s)
      Tsukasa Shigehiro, Wenjia Zhai, Arun Vaidyanath, Junko Masuda, Akifumi Mizutani, Tomonari Kasai, Hiroshi Murakami, Hiroki Hamada, David S Salomon, Katsuhiko Mikuni, Yuhki Seno, Tadakatsu Mandai, Masaharu Seno
    • Journal Title

      Journal of Microencapsulation

      Volume: 33 Issue: 2 Pages: 172-182

    • DOI

      10.3109/02652048.2016.1144815

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] ADAM12-cleaved ephrin-A1 contributes to lung metastasis.2014

    • Author(s)
      Ieguchi K, Tomita T, Omori T, Komatsu A, Deguchi A, Masuda J, Duffy SL, Coulthard MG, Boyd A, Maru Y.
    • Journal Title

      Oncogene

      Volume: 未定 Issue: 17 Pages: 2179-90

    • DOI

      10.1038/onc.2013.180

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Golgin Tether Giantin Regulates the Secretory Pathway by Controlling Stack Organization within Golgi Apparatus2013

    • Author(s)
      Mayuko Koreishi, Thomas J. Gniadek, Sidney Yu, Junko Masuda, Yasuko Honjo, Ayano Satoh
    • Journal Title

      PLOS One

      Volume: 8 Issue: 3 Pages: 1-10

    • DOI

      10.1371/journal.pone.0059821

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 化学物質のがん幹細胞誘導性に関するin vitroにおける簡易評価技術の開発2015

    • Author(s)
      佐々田 沙紀、星川 健太、松本 拓馬、Sanchez Calle Anna、Vaidyanath Arun、増田 潤子、笠井 智成、妹尾 昌治
    • Organizer
      日本動物実験代替法学会 第28回大会
    • Place of Presentation
      横浜
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 化学物質のがん幹細胞誘導性評価において iPS 細胞を用いる技術の開発2015

    • Author(s)
      笠井 智成、佐々田 沙紀、星川 健太、松本 拓馬、増田 潤子、Anna Sanchez Calle、 Arun Vaidyanath、工藤 孝幸、妹尾 昌治
    • Organizer
      日本動物実験代替法学会 第28回大会
    • Place of Presentation
      横浜
    • Year and Date
      2015-12-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] マウスiPS細胞由来がん幹細胞におけるダウノルビシンによるp53経路の活性化2015

    • Author(s)
      相澤 一輝、宗田 龍幸、谷口 早紀、尾上 稜馬、村上 宏、増田 潤子、Vaidyanath Arun、笠井 智成、水谷 昭文、妹尾 昌治
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] がん幹細胞ニッチでのがん幹細胞自己複製促進2015

    • Author(s)
      恩賀 咲、平本 祐樹、松田 修一、村上 宏、増田 潤子、Vaidyanath Arun、笠井 智成、水谷 昭文、妹尾 昌治
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] がん幹細胞miPS-LLCcmが形成する微小環境によるNanogタンパク質の局在変化2015

    • Author(s)
      公文 一輝、本山 大暉、増田 潤子、Marta Prieto Vila、相澤 一輝、尾上 稜馬、笠井 智成、村上 宏、水谷 昭文、妹尾 昌治
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] シグナル伝達阻害剤によるがん幹細胞誘導メカニズムの解析2015

    • Author(s)
      佐々田 沙紀、星川 健太、松本 拓馬、Anna Sanchez Calle、水谷 昭文、Arun Vaidyanath、増田 潤子、笠井 智成、妹尾 昌治
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] G-CSF刺激依存の好中球分化誘導におけるGab2タンパク質の役割2015

    • Author(s)
      趙 香琳、河野 俊一郎、笠井 智成、増田 潤子、水谷 昭文、妹尾 昌治、村上 宏
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] がんモデル動物を用いた悪性度の違いによる全身免疫能変化の解析2015

    • Author(s)
      増田 潤子、高山 英次、佐藤 あやの、守本 祐司、本庶 仁子、石塚 俊晶、徳野 慎一、青笹 季文、光吉 俊二、重廣 司、前野 成実、村上 宏、笠井 智成、水谷 昭文、Arun Vaidyanath、妹尾 彬正、川木 晴美、神谷 真子、近藤 信夫、一瀬 雅夫、一戸 辰夫、妹尾 昌治
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Systemic Immunities on Mouse with Malignant Cancer.2015

    • Author(s)
      Masuda J, Takayama E, Seno M.
    • Organizer
      Frontiers in Basic Immunology 2015
    • Place of Presentation
      ベセスダ (アメリカ)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 糖修飾ドセタキセル封入リポソームを用いたドラッグデリバリー2015

    • Author(s)
      重廣 司、増田 潤子、 水谷 昭文、 笠井 智成、 村上 宏、 濱田 博喜、 伊藤 哲也、 萬代 忠勝、 妹尾 昌治
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Dextran sodium sulfate-induced colitis in dendritic cell deletion mice.2015

    • Author(s)
      Masuda J, Kitani A, Fuss I, Seno M, Strober W.
    • Organizer
      Annual Meeting 2015, American Association for Cancer Research (AACR)
    • Place of Presentation
      フィラデルフィア (アメリカ)
    • Year and Date
      2015-04-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of cancer stem-like cells derived from mouse induced pluripotent stem cells transformed by tumor-derived exosomes/microvesicles2014

    • Author(s)
      Junko Masuda
    • Organizer
      AACR Annual Meeting
    • Place of Presentation
      サンディエゴ
    • Related Report
      2013 Research-status Report
  • [Book] 次世代のがん治療薬・診断のための研究開発2016

    • Author(s)
      笠井 智成、Arun Vaidyanath、妹尾 彬正、朝倉 真実、増田 潤子、水谷 昭文、村上 宏、妹尾 昌治
    • Total Pages
      400
    • Publisher
      技術情報協会
    • Related Report
      2015 Annual Research Report
  • [Book] Biology in Stem Cell Niche2015

    • Author(s)
      Mizutani A, Yan T, Vaidyanath A, Masuda J, Seno A, Kasai T, Murakami H, Seno M
    • Total Pages
      226
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2022-01-27  

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