Quantitative evaluation and imaging of intratumoral oxygen metabolism by O-15 oxygen PET

• Project/Area Number: 25861095
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Osaka University
• Principal Investigator: WATABE Tadashi 大阪大学, 医学(系)研究科(研究院), 寄附講座助教 (90648932)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 腫瘍 / 低酸素 / 酸素代謝 / 血流 / PET / FMISO / O15 / O-15ガス

Outline of Final Research Achievements

The purpose of this study was to evaluate the intratumoral perfusion and oxygen metabolism in hypoxic regions in the rat xenograft model. Six male Fischer rats with C6 glioma were investigated with 18F-FMISO PET and steady state inhalation method of 15O-gas PET. The tumoral blood flow (TBF), tumoral metabolic rate of oxygen (TMRO2), oxygen extraction fraction (OEF), and tumoral blood volume (TBV) were measured under artificial ventilation with 15O-CO2, 15O-O2, and 15O-CO gases. Intratumoral hypoxic regions showed significantly lower TBF, higher OEF, lower TMRO2, and lower TBV values than non-hypoxic regions. This study demonstrated intratumoral hypoxic regions showed decreased blood flow with an increased OEF, suggesting the need for a treatment strategy to normalize the blood flow for oxygen-avid active tumor cells in hypoxic regions.

Research Products

• [Journal Article] CBF/CBV Maps in Normal Volunteers Studied with 15O PET: A Possible Index of Cerebral Perfusion Pressure. (2014)
  • Author(s): Tadashi Watabe, Eku Shimosegawa, Hiroki Kato, Kayako Isohashi, Mana Ishibashi, Jun Hatazawa
  • Journal Title: Neuroscience Bulletin Volume: (in press)
  • Peer Reviewed
• [Journal Article] Paradoxical Reduction of Cerebral Blood Flow after Acetazolamide Loading: a Hemodynamic and Metabolic Studies with 15O-PET (2014)
  • Author(s): Tadashi Watabe, Eku Shimosegawa, Hiroki Kato, Kayako Isohashi, Mana Ishibashi, Kazuo Kitagawa, Toshiyuki Fujinaka, Toshiki Yoshimine, Jun Hatazawa
  • Journal Title: Neuroscience Bulletin Volume: (in press)
  • Peer Reviewed
• [Journal Article] Development of an ultrahigh resolution Si-PM based PET system for small animals. (2014)
  • Author(s): Yamamoto S, Watabe H, Kanai Y, Watabe T, Kato K, Hatazawa J.
  • Journal Title: Phys Med Biol. Volume: 58(21) Issue: 21 Pages: 7875-88
  • DOI: 10.1088/0031-9155/58/21/7875
  • Peer Reviewed
• [Presentation] 腫瘍内低酸素領域の酸素代謝の定量評価：18F-FMISO and 15O-Gas PET (2014)
  • Author(s): 渡部直史、堀次元気、金井泰和、池田隼人、加藤弘樹、花岡宏平、仲定弘、礒橋佳也子、下瀬川恵久、畑澤順
  • Organizer: 第54回日本核医学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-11-06 – 2014-11-08
• [Presentation] Quantitative PET imaging of intratumoral oxygen metabolism in hypoxic lesions: comparison study of 18F-FMISO and 15O-labeled gas inhalation PET. (2014)
  • Author(s): Watabe T, Kanai Y, Ikeda H, Horitsugi G, Matsunaga K, Kato H, Isohashi K, Abe K, Shimosegawa E, Hatazawa J
  • Organizer: Annual Congress of the European Association of Nuclear Medicine (EANM 2014)
  • Place of Presentation: Gothernburg, Sweden
  • Year and Date: 2014-10-18 – 2014-10-23
• [Presentation] Quantitative PET imaging of intratumoral oxygen metabolism by 15O-labeled gas inhalation PET. (2014)
  • Author(s): Watabe T, Horitsugi G, Kanai Y, Ikeda H, Kato H, Hanaoka K, Naka S, Isohashi K, Shimosegawa E, Hatazawa J
  • Organizer: Society of Nuclear Medicine and Molecular Imaging's 61th Annual Meeting (SNMMI 2014)
  • Place of Presentation: St Louis, USA
  • Year and Date: 2014-06-07 – 2014-06-12
• [Presentation] 15O標識ガス吸入PETによる腫瘍内酸素代謝の定量評価 (2014)
  • Author(s): 渡部直史、堀次元気、金井泰和、池田隼人、加藤弘樹、花岡宏平、仲定弘、礒橋佳也子、下瀬川恵久、畑澤順
  • Organizer: 第9回日本分子イメージング学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-05-22 – 2014-05-23
• [Presentation] Quantitative PET imaging of intratumoral oxygen metabolism by 15O-labeled gas inhalation PET (2014)
  • Author(s): Tadashi Watabe; Genki Horitsugi; Yasukazu Kanai; Hayato Ikeda; Hiroki Kato; Kohei Hanaoka; Sadahiro Naka; Kayako Isohashi; Eku Shimosegawa; Jun Hatazawa
  • Organizer: SNMMI2014
  • Place of Presentation: セントルイス(アメリカ)
• [Presentation] 15O標識ガス吸入PETによる腫瘍内酸素代謝の定量評価 (2014)
  • Author(s): 渡部直史、堀次元気、金井泰和、池田隼人、加藤弘樹、 花岡宏平、仲定弘、礒橋佳也子、下瀬川恵久、畑澤順
  • Organizer: 分子イメージング学会2014
  • Place of Presentation: 大阪
• [Presentation] Quantitative evaluation of myocardial blood flow and oxygen metabolism in the normal anesthetized rat: 15O-labeled gas inhalation PET. (2013)
  • Author(s): Tadashi Watabe; Hiroshi Watabe; Yasukazu Kanai; Eku Shimosegawa; Kohei Hanaoka; Genki Horitsugi; Kayako Isohashi; Hiroki Kato; Mitsuaki Tatsumi; Jun Hatazawa
  • Organizer: SNMMI2013
  • Place of Presentation: バンクーバー(カナダ)
• [Presentation] O-15 ガス PET を用いた腫瘍内酸素代謝のイメージングと定量評価 (2013)
  • Author(s): 渡部直史
  • Organizer: 第3回核医学画像解析研究会
  • Place of Presentation: 秋田
• [Presentation] 15O標識ガス定常吸入法による ラット脳血流・酸素代謝評価 (2013)
  • Author(s): 渡部直史
  • Organizer: 第9回小動物インビボイメージング研究会
  • Place of Presentation: 福井
• [Book] Nuclear Oncology (2014)
  • Author(s): Masahiro Yanagawa, Noriyuki Tomiyama, Tadashi Watabe, Kayako Isohashi, Jun Hatazawa
  • Total Pages: 16
  • Publisher: Springer
• [Remarks] 大阪大学大学院医学系研究科附属PET分子イメージングセンター
  • URL: http://www.pet.med.osaka-u.ac.jp/
• [Remarks] 大阪大学医学系研究科附属PET分子イメージングセンター
  • URL: http://www.pet.med.osaka-u.ac.jp