| Project/Area Number |
25861161
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
SAKAI Yusuke 長崎大学, 医歯薬学総合研究科(医学系), 助教 (10608904)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 肝細胞 / 細胞シート / 再生医療 / 血管新生 |
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| Outline of Final Research Achievements |
Subcutaneous liver tissue engineering is an attractive and minimally invasive approach as liver regenerative medicine. In this study, we describe a subcutaneous human liver construction allowing for rapidly vascularized grafts by transplanting engineered hepatocyte/fibroblast sheets (EHFSs).
EHFSs were thicker than hepatocyte-only sheets (HSs), this result could aide in easy manipulation during transplantation. The EHFSs showed superior expression levels of vascularization-associated growth factors (VEGF, TGFb1, and HGF) in vitro. EHFSs were transplanted subcutaneously into immunodeficient mice. The EHFSs formed vascularized subcutaneous human liver tissues (VSLTs) by rapidly neo-vascularization under mouse skin. Furthermore, subacute hepatic failure model mice with VSLT were alive at least 7 weeks after liver damage. This approach could prove valuable for establishing novel cell therapies for liver diseases.
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