Organ reengineering of a small bowel graft using decellularized scaffold of porcine intestine and intestinal organoid

• Project/Area Number: 25861164
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Keio University
• Principal Investigator: Hibi Taizo 慶應義塾大学, 医学部, 講師 (10338072)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 移植外科学 / 小腸グラフト / 脱細胞化骨格

Outline of Final Research Achievements

We aimed to develop a transplantable small bowel graft with the organ decellularization technique using porcine intestinal scaffold and intestinal organoid as a future treatment alternative for intestinal failure patients waiting for small bowel transplantation. After the decellularization technique of porcine intestine matured, we switched the plan to use rat intestine because of logistic reasons. We successfully reperfused the small bowel graft through the vessels with heparinized saline and confirmed that there is no cell nuclei within the scaffold by immunohistochemical staining. We are currently conducting precise observation of the internal structure of the graft using electron microscope and evaluation of DNA included in the cytoskeleton and the nature of extracellular matrix.

Research Products

• [Presentation] 難治がんの治療戦略：腫瘍外科と移植外科の融合 (2015)
  • Author(s): 日比 泰造
  • Organizer: 第12回CAESUR Seminar
  • Place of Presentation: 新宿文化クイントビル（東京都新宿区）
  • Year and Date: 2015-12-19
  • Invited
• [Presentation] 難治がんの温故知新：腫瘍外科と移植外科の融合 (2015)
  • Author(s): 日比 泰造
  • Organizer: 第6回東京周術期管理講演会
  • Place of Presentation: ハイアットリージェンシー東京（東京都新宿区）
  • Year and Date: 2015-11-11
  • Invited
• [Presentation] Outcomes of living donors in small bowel transplantation (2015)
  • Author(s): Taizo Hibi
  • Organizer: XIV International Small Bowel Transplantation Symposium
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2015-06-12
  • Int'l Joint Research
• [Presentation] Outcomes of living donors in small bowel transplantation (2015)
  • Author(s): Taizo Hibi
  • Organizer: XIV International Small Bowel Transplant Symposium
  • Place of Presentation: BUENOS AIRES, ARGENTINA
  • Year and Date: 2015-06-12
• [Presentation] 生体小腸移植における成人ドナーの術後成績 (2015)
  • Author(s): 日比 泰造
  • Organizer: 第27回日本小腸移植研究会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-14
• [Presentation] Long-term follow-up of adult living donors for small bowel transplant in pediatric recipients (2013)
  • Author(s): Taizo Hibi, Masahiro Shinoda, Osamu Itano, Minoru Kitago, Hiroshi Yagi, Yuta Abe, Ken Hoshino, Akihiro Fujino, Naoki Shimojima, Koji Tomita, Tatsuo Kuroda, Minoru Tanabe, Yuko Kitagawa
  • Organizer: XIII International Small Bowel Transplant Symposium
  • Place of Presentation: Oxford, United Kingdom