Analysis of Homeobox genes and specific peptide for molecular therapy in Glioblastoma

Research Project

Project/Area Number	25861269
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Neurosurgery
Research Institution	Nagoya University
Principal Investigator	HASEGAWA Hitoki 名古屋大学, 医学(系)研究科(研究院), 研究員 (10454381)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	神経膠芽腫 / ホメオボックス / Notch / PRRX1 / 浸潤 / 転移 / EMT / ホメオボックスタンパク質 / ホメオタンパク質
Outline of Final Research Achievements	The expression levels of PRRX1 mRNA were increased in most of the low grade and high-grade glioma samples when compared to the levels in the normal brain tissues. The depression of PRRX1 suppressed the invasion and neurosphere formation of glioblastoma cells. We also found specific activation of the Notch pathway in PRRX1-expressing cells. Consistent with the activation of Notch signaling, increased levels of HES1 mRNA and protein by PRRX1 expression were observed. We found a correlation between PRRX1 and HES1. Our results indicate that the activation of Notch signaling by PRRX1 is associated with the promotion of glioblastoma cell invasion and tumorigenicity.

Report

(3 results)