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A study of optimal conditions for preservation of synovial tissues and synovium-derived mesenchymal stem cells

Research Project

Project/Area Number 25861299
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Horie Masafumi  東京医科歯科大学, 再生医療研究センター, 助教 (90622647)

Research Collaborator Sekiya Ichiro  東京医科歯科大学, 再生医療研究センター, 教授
Mizuno Mitsuru  東京医科歯科大学, 再生医療研究センター, 助教
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords滑膜間葉系幹細胞 / 滑膜幹細胞
Outline of Final Research Achievements

Synovium-derived mesenchymal stem (synovial-MSCs) cells are one of the attractive cell sources for cartilage and meniscal regeneration. Because the number of cell processing center is limited in our country, it is essential to preserve and transport fresh tissues and/or the cell product for spreading our stem cell treatment to worldwide. In the present study, we explored the optimal conditions for preservation of tissues and cell products using synovial tissues which were obtained at total knee arthroplasty. The results demonstrated that the optimal temperature for preservation of synovial tissues was between at 13 to 18 °C. Also, preserving synovial-MSCs in human serum at 13 °C was the best condition for cell viability, colony-forming ability, cell proliferation ability and chondrogenic ability. Our finding will help us to establish basic technologies of our stem cell therapy designed for global usage.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (15 results)

All 2016 2015 2014 2013

All Journal Article (12 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Invited: 3 results)

  • [Journal Article] Not single but periodic injections of synovial mesenchymal stem cells maintain viable cells in knees and inhibit osteoarthritis progression in rats.2016

    • Author(s)
      Ozeki N, Muneta T, Koga H, Nakagawa Y, Mizuno M, Tsuji K, Mabuchi Y, Akazawa C, Kobayashi E, Matsumoto K, Futamura K, Saito T, Sekiya I
    • Journal Title

      Osteoarthritis Cartilage

      Volume: in press Issue: 6 Pages: 1061-70

    • DOI

      10.1016/j.joca.2015.12.018

    • NAID

      40021330438

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Synovial mesenchymal stem cells promote meniscus regeneration augmented by an autologous Achilles tendon graft in a rat partial meniscus defect model.2015

    • Author(s)
      Ozeki N, Muneta T, Matsuta S, Koga H, Nakagawa Y, Mizuno M, Tsuji K, Mabuchi Y, Akazawa C, Kobayashi E, Saito T, Sekiya I.
    • Journal Title

      Stem Cells.

      Volume: 33(6) Issue: 6 Pages: 1927-38

    • DOI

      10.1002/stem.2030

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 3.Arthroscopic transplantation of synovial stem cells improves clinical outcomes in knees with cartilage defects.2015

    • Author(s)
      Ichiro Sekiya, Takeshi Muneta, Masafumi Horie, Hideyuki Koga
    • Journal Title

      Clin Orthop Relat Res.

      Volume: 473 Issue: 7 Pages: 2316-2326

    • DOI

      10.1007/s11999-015-4324-8

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mouse synovial mesenchymal stem cells increase in yield with knee inflammation.2015

    • Author(s)
      Matsukura Y, Muneta T, Tsuji K, Miyatake K, Yamada J, Abula K, Koga H, Tomita M, Sekiya I.
    • Journal Title

      J Orthop Res.

      Volume: 33 Issue: 2 Pages: 246-53

    • DOI

      10.1002/jor.22753

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Mesenchymal stem cells in synovial fluid increase after meniscus injury.2014

    • Author(s)
      Matsukura Y, Muneta T, Tsuji K, Koga H, Sekiya I
    • Journal Title

      Clin Orthop Relat Res.

      Volume: 472 Issue: 5 Pages: 1357-1364

    • DOI

      10.1007/s11999-013-3418-4

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Meniscus regeneration by syngeneic, minor mismatched, and major mismatched transplantation of synovial mesenchymal stem cells in a rat model.2014

    • Author(s)
      Okuno M, Muneta T, Koga H, Ozeki N, Nakagawa Y, Tsuji K, Yoshiya S, Sekiya I.
    • Journal Title

      J Orthop Res.

      Volume: in press Issue: 7 Pages: 928-36

    • DOI

      10.1002/jor.22614

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Repetitive allogeneic intraarticular injections of synovial mesenchymal stem cells promote meniscus regeneration in a porcine massive meniscus defect model2014

    • Author(s)
      Hatsushika D, Muneta T, Nakamura T, Horie M, Koga H, Nakagawa Y, Tsuji K, Hishikawa S, Kobayashi E, Sekiya I
    • Journal Title

      Osteoarthritis Cartilage

      Volume: in press Issue: 7 Pages: 941-50

    • DOI

      10.1016/j.joca.2014.04.028

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 滑膜由来間葉系幹細胞を利用した半月板再生技術2013

    • Author(s)
      堀江雅史 関矢一郎 宗田大
    • Journal Title

      骨研究最前線

      Volume: 3 Pages: 207-215

    • Related Report
      2013 Research-status Report
  • [Journal Article] 滑膜由来間葉系幹細胞を用いた半月板再生の基礎と臨床への展望2013

    • Author(s)
      堀江雅史 関矢一郎 宗田大
    • Journal Title

      整形・災害外科

      Volume: 56.5 Pages: 593-601

    • Related Report
      2013 Research-status Report
  • [Journal Article] 滑膜由来間葉系幹細胞による膝半月板再生2013

    • Author(s)
      堀江雅史 関矢一郎 宗田大
    • Journal Title

      免疫と臨床

      Volume: 21 Pages: 148-156

    • Related Report
      2013 Research-status Report
  • [Journal Article] Transplantation of aggregates of synovial mesenchymal stem cells regenerates meniscus more effectively in a rat massive meniscal defect2013

    • Author(s)
      Katagiri H, Muneta T, Tsuji K, Horie M, Koga H, Ozeki N, Kobayashi E, Sekiya I.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 435 Issue: 4 Pages: 603-609

    • DOI

      10.1016/j.bbrc.2013.05.026

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Intraarticular Injection of Synovial Stem Cells Promotes Meniscal Regeneration in a Rabbit Massive Meniscal Defect Model.2013

    • Author(s)
      Hatsushika D.
    • Journal Title

      JOURNAL OF ORTHOPAEDIC RESEARCH

      Volume: In Press Issue: 9 Pages: 1354-1359

    • DOI

      10.1002/jor.22370

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Synovial stem cell therapy for meniscal regeneration2015

    • Author(s)
      堀江 雅史
    • Organizer
      ISAKOS
    • Place of Presentation
      Lyon, France
    • Year and Date
      2015-06-07 – 2015-06-11
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 滑膜幹細胞を用いた半月板再生2014

    • Author(s)
      堀江 雅史
    • Organizer
      日本関節病学会
    • Place of Presentation
      東京
    • Year and Date
      2014-11-06 – 2014-11-07
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 滑膜幹細胞を用いた半月板再生2014

    • Author(s)
      堀江 雅史
    • Organizer
      JOSKAS
    • Place of Presentation
      広島
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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