A study of optimal conditions for preservation of synovial tissues and synovium-derived mesenchymal stem cells
| Project/Area Number |
25861299
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
Horie Masafumi 東京医科歯科大学, 再生医療研究センター, 助教 (90622647)
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| Research Collaborator |
Sekiya Ichiro 東京医科歯科大学, 再生医療研究センター, 教授
Mizuno Mitsuru 東京医科歯科大学, 再生医療研究センター, 助教
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 滑膜間葉系幹細胞 / 滑膜幹細胞 |
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| Outline of Final Research Achievements |
Synovium-derived mesenchymal stem (synovial-MSCs) cells are one of the attractive cell sources for cartilage and meniscal regeneration. Because the number of cell processing center is limited in our country, it is essential to preserve and transport fresh tissues and/or the cell product for spreading our stem cell treatment to worldwide. In the present study, we explored the optimal conditions for preservation of tissues and cell products using synovial tissues which were obtained at total knee arthroplasty. The results demonstrated that the optimal temperature for preservation of synovial tissues was between at 13 to 18 °C. Also, preserving synovial-MSCs in human serum at 13 °C was the best condition for cell viability, colony-forming ability, cell proliferation ability and chondrogenic ability. Our finding will help us to establish basic technologies of our stem cell therapy designed for global usage.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Not single but periodic injections of synovial mesenchymal stem cells maintain viable cells in knees and inhibit osteoarthritis progression in rats.2016
Author(s)
Ozeki N, Muneta T, Koga H, Nakagawa Y, Mizuno M, Tsuji K, Mabuchi Y, Akazawa C, Kobayashi E, Matsumoto K, Futamura K, Saito T, Sekiya I
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Journal Title
Osteoarthritis Cartilage
Volume: in press
Issue: 6
Pages: 1061-70
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Synovial mesenchymal stem cells promote meniscus regeneration augmented by an autologous Achilles tendon graft in a rat partial meniscus defect model.2015
Author(s)
Ozeki N, Muneta T, Matsuta S, Koga H, Nakagawa Y, Mizuno M, Tsuji K, Mabuchi Y, Akazawa C, Kobayashi E, Saito T, Sekiya I.
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Journal Title
Stem Cells.
Volume: 33(6)
Issue: 6
Pages: 1927-38
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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