Approach from muscle to bone and cartilage regeneration and development of the treatment of diseases

• Project/Area Number: 25861347
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kinki University
• Principal Investigator: YANO Masato 近畿大学, 医学部, 助教 (30500821)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 破骨細胞 / 筋芽細胞 / 異所性骨化 / 進行性骨化性線維異形成症 / TGF-β / 破骨細胞分化

Outline of Final Research Achievements

We investigated the role of osteoclasts during heterotopic bone formation in muscle tissue. The implantation of myoblasts with BMP-2 induced robust heterotopic ossification with an increase in the formation of osteoclasts in the muscle tissues than subcutaneous tissues. Moreover, the causal mutation transfection of fibrodysplasia ossification progressive in myoblasts enhanced the formation of osteoclasts through TGF-β in vivo and in vitro. This study demonstrated that the causal mutation transfection of fibrodysplasia ossification progressive in myoblasts enhances bone and cartilage regeneration through formation of osteoclasts from its precursor in muscle tissues.

Research Products

• [Journal Article] Fibrodysplasia ossificans progressiva-related activated activin-like kinase signaling enhances osteoclast formation during heterotopic ossification in muscle tissues. (2014)
  • Author(s): Yano M, Kawao N, Okumoto K, Tamura Y, Okada K, Kaji H
  • Journal Title: J Biol Chem Volume: 289 Issue: 24 Pages: 16966-16977
  • DOI: 10.1074/jbc.m113.526038
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Role of matrix metalloproteinase-10 in the BMP-2 inducing osteoblastic differentiation (2013)
  • Author(s): Mao L, Yano M, Kawao N, Tamur a Y, Okada K, Kaji H
  • Journal Title: Endocrine Journal Volume: 60 Issue: 12 Pages: 1309-1319
  • DOI: 10.1507/endocrj.EJ13-0270
  • NAID: 130004770406
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed
• [Presentation] 活性化ALK(activin-like kinase)2 シグナルは筋骨化過程における破骨細胞の形成を促進する. (2014)
  • Author(s): 矢野 昌人, 河尾 直之, 奥本 勝美, 田村 行識, 岡田 清孝, 梶 博史
  • Organizer: 第32回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪市北区中之島）
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] 進行性骨化性線維異形成症の原因変異より誘導される新規因子Tmem176bは筋芽細胞から骨芽細胞への分化を促進する． (2014)
  • Author(s): 矢野昌人、河尾直之、田村行識、岡田清孝、梶博史
  • Organizer: 第32回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪市北区中之島）
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] MMP-10はBMP-2による筋芽細胞から骨芽細胞への分化を促進する． (2014)
  • Author(s): 岡田清孝、毛莉、矢野昌人、田村行識、河尾直之、梶博史
  • Organizer: 第32回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪市北区中之島）
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] 筋骨化シグナルにより誘導される新規因子 Tmem176b は筋芽細胞 から骨への分化を促進する (2013)
  • Author(s): 矢野昌人、河尾直之、田村行識、岡清孝、梶博史
  • Organizer: 第１０６回近畿生理学談話会
  • Place of Presentation: 奈良（奈良県立医科大学）
• [Remarks]
  • URL: http://www.med.kindai.ac.jp/physio2/