| Project/Area Number |
25861363
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 局所麻酔薬 / 脊髄 / パッチクランプ / QX-314 / 脊髄後角 / 痛み |
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| Outline of Final Research Achievements |
I examined the effects of QX-314 on rat dorsal horn neurons, where primary afferents terminate. First, I used whole-cell patch clamp recording from the dorsal horn neurons of transverse spinal cord slices. QX-314 decreased the amplitude of N-methyl-D-asparate induced currents and of the exitatory postsynaptic currents evoked by the dorsal root stimulation. QX-314, however, affected neither the frequency nor the amplitude of spontaneous excitatory postsynaptic current. Then, I used in vivo extracellular recording from the rat spinal cord. QX-314 decreased the frequency of action potentials induced by noxious stimulation to the rat hindpaw, especially in nociception specific neurons. These results suggest that QX-314 may act as an analgesic drug by inhibition of excitatory responses in dorsal horn neurons.
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