The study to evaluate the effect of bumetanide on the effectiveness of anesthetic agents in the neonate

Research Project

Project/Area Number	25861389
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Anesthesiology
Research Institution	Yokohama City University
Principal Investigator	KARIYA TAKAYUKI 横浜市立大学, 附属市民総合医療センター, 助教 (90614404)
Project Period (FY)	2013-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	鎮静作用 / NKCC1 / KCC2 / 麻酔薬 / 幼若脳 / ブメタニド / GABA / GABAA受容体
Outline of Final Research Achievements	Cl- transporters control intracellular Cl- concentration, which affects the behaviour of anesthetic agents. We examined if the activation of KCC2 enhances the sedative actions of midazolam. We revealed that CLP290, KCC2 enhancer via phosphorylation of KCC2, enhanced MDZ-dependent sedation.