Anti-inflammatory effect of intravenous injection of adipose-derived regenerative cells using a cystitis rat model

• Project/Area Number: 25861417
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Nagoya University
• Principal Investigator: FUNAHASHI Yasuhito 名古屋大学, 医学部附属病院, 助教 (70534824)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 脂肪由来幹細胞 / 膀胱 / 炎症 / homing / ラット / ホーミング / 間質性膀胱炎 / 膀胱炎モデル / 過活動膀胱

Outline of Final Research Achievements

We injected adipose-derived regenerative cells (ADRCs) via the tail vein using a cystitis rat model. Near-infrared light in vivo imaging showed "homing phenomenon" of ADRCs to the inflamed bladder. Pencil lens charge-coupled device microscopy showed increased microcirculation of the bladder wall in the ADRCs-injected rats. There were no obvious histological changes in the bladder, and a significant decrease of pro-inflammatory cytokines by ELISA. Cystometrogram revealed prolonged intercontraction intervals. Nociceptive behaviors decreased in the ADRCs-injected group. The results of this study indicated the possibility of intravenous injection of ADRCs as a new treatment for interstitial cystitis.