Development of an in vitro culture methods for low quality embryos

Research Project

Project/Area Number	25861479
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Obstetrics and gynecology
Research Institution	University of Toyama
Principal Investigator	NISHIZONO Hirofumi 富山大学, 生命科学先端研究センター, 助教 (10502289)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords	胚培養 / メタボローム / 卵子品質 / ミトコンドリア / メタボロミクス
Outline of Final Research Achievements	We are interested in developing a prophylactic method for oocyte aging. To compare small molecule metabolites and embryo quality, we used embryos from C57BL/6J mice (as a model of high quality embryo) and DBA/2J mice (as a model of low quality embryo). Our results showed that the amount of specific metabolites associated with amino acids contained in DBA/2J embryos are lower than the amount of metabolites contained in C57BL/6J embryos. Also we found that the developmental rate of DBA/2J embryos were improved using novel medium including these specific metabolites.

Report

(3 results)