| Project/Area Number |
25861508
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Juntendo University |
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Principal Investigator |
Kuroda Keiji 順天堂大学, 医学部, 准教授 (60459162)
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| Research Collaborator |
OZAKI Rie 順天堂大学, 医学部, 大学院生
OCHIAI Asako 順天堂大学, 医学部, 大学院生
IKEMOTO Yuko 順天堂大学, 医学部, 大学院生
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 子宮内膜 / 脱落膜化 / 妊娠 / 習慣流産 / レチノイド / 子宮NK細胞 / レスベラトロール / サーチュイン / 子宮内膜間質細胞 / 子宮内膜脱落膜化 / 細胞分化 / レチノイン酸 / グルココルチコイド |
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| Outline of Final Research Achievements |
In pregnancy, decidual transformation of human endometrial stromal cells (HESCs) is essential for embryo implantation. Uterine natural killer (uNK) cells in HESCs induce angiogenesis at implantation, but aberrant elevated uNK cell levels are associated with reproductive failure including recurrent pregnancy loss. We identified that 11βHSD1/GR/MR signaling pathway regulated uNK cells and MR-dependent retinoid (vitamin A) metabolism pathway in primary HESC cultures decidualized in vitro. The wholesale reprogramming of the retinoid signaling, transport, and metabolism pathways in decidualizing HESCs may be critical in limiting exposure of the implanting conceptus in early pregnancy. Furthermore, optimal decidual transformation of primary cultures may involve not only cell differentiation also apoptosis or senescence of specific subpopulations.
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