| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Sensory hair cells and auditory neurons do not regenerate throughout life, and loss of these cells is irreversible and cumulative. However recent advances in stem cell biology have provided hope that stem cell therapy will come closer to regenerating sensory hair cells and auditory neurons in humans. In the present study, we have investigated the effects of sodium valproate on the fate of embryonic stem cells. Consequently sodium valproate has clearly demonstrated to promote neurogenesis of ES cells. Thereafter we performed the comprehensive gene expression analysis of ES cells during differentiation into inner ear progenitor cells. Furthermore. to establish the methods for selecting inner ear progenitor cells exclusively, we have integrated GFP, a reporter gene, into the Tlx3 allele of the ES cell lines.
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