Development of new detection method of AMD through the unbalance of compliment factors

• Project/Area Number: 25861646
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: YONEDA Kazuhito 京都府立医科大学, 医学(系)研究科(研究院), 助教 (00347460)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 加齢黄斑変性 / 補体 / マクロファージ / 眼病理学

Outline of Final Research Achievements

To develop a new method of early detection of AMD, we did immunohistochemistry of various compliment factors in the human donor eyes that include both normal eyes and AMD patients eyes. We also developed the new Ex Vivo evaculation method of the pharmacological effects of some seed chemicals. We found the new effect of GRA family chemicals that downregulated the TNF production of the LPS stimulated macrophages. In addition, we also analysed the serum from some kinds of posterior ocular inflammation disease patients including AMD about 53 kinds of cytokines and 8 kinds of complement inhibitory factors. We choose ten useful parameter cytokines to develop the new early detection
method of AMD.