| Project/Area Number |
25861675
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatric surgery
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| Research Institution | National Center for Child Health and Development |
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Principal Investigator |
Hirano Hiroshi 国立研究開発法人国立成育医療研究センター, 移植免疫研究室, 共同研究員 (00414334)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | がん / 樹状細胞 / 腫瘍免疫学 / iPS細胞 / 再生医療 |
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| Outline of Final Research Achievements |
First, this research developed a differentiation method of human iPS cells-derived dendritic cells (human iPS-DC). Based on the method to generate murine iPS-DC, which we reported in a previous work, we established the method to generate the dendritic cells from human iPS cells by the co-culture with OP9 cells, which is one of stromal cell lines. In this process, we established a new method to generate murine iPS-DC. This method needs less period than the previous methods. The investigation of the mechanism and the application of this new technology to human iPS cells will be issues to be addressed in the future.
Second, we developed a method to prepare tumor-bearing model mice by the injection of a melanoma cell line, to investigate a new treatment method of cancer by the combination of the iPS-DC injection and the radiation therapy. However, we could not verify the therapeutic effect.
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