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Research on cytological characteristics of human nail tissue for regenerating nail matrix

Research Project

Project/Area Number 25861680
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plastic surgery
Research InstitutionTokyo Medical and Dental University

Principal Investigator

USAMI Satoshi  東京医科歯科大学, 医学部附属病院, 医員 (10635577)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords爪ケラチノサイト / 硬ケラチン / サイトカイン / 爪培養 / ケラチノサイト / 細胞増殖曲線 / サイトケラチン
Outline of Final Research Achievements

We established cell separation, primary and passage culture methods of nail and skin keratinocytes harvested from polydactyly patients. Next, we examined the effects of various stimulating factors on the functions of these keratinocytes and hard keratins. Nail and skin keratinocytes showed similar cell characteristics, however, the nail keratinocytes differentiated and cornified earlier losing their ability to undergo cell division. Basal FGF and EGF proliferated keratinocytes at a low concentration (0.1-5ng/ml), and inhibited their growth at a high concentration. On the one hand, Vitamin D showed concentration-dependent inhibition. With regard to the production of hard keratin in a nail keratinocyte monolayer culture, Vitamin D group showed higher production; bFGF and EGF increased the production in a concentration-dependent manner.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (1 results)

All 2015

All Presentation (1 results)

  • [Presentation] ヒト爪ケラチノサイトの細胞学的特性および各種刺激因子の検討~第1報~2015

    • Author(s)
      宇佐美聡、植村法子、山本真魚、森弘樹、岡崎睦
    • Organizer
      第7回日本創傷外科学会
    • Place of Presentation
      東京都文京区東京ドームホテル
    • Year and Date
      2015-07-24 – 2015-07-25
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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