Photodynamic therapy for multiple-drug resistant Pseudomonas aeruginosa using 5-aminolevulinic acid
| Project/Area Number |
25861694
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Osaka City University |
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Principal Investigator |
SAKAHARA Daisuke 大阪市立大学, 医学(系)研究科(研究院), 研究員 (30647912)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 緑膿菌 / 光線力学療法 / 5-アミノレブリン酸 / EDTA / 5-ALA |
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| Outline of Final Research Achievements |
Total porphyrin production was increased when P. aeruginosa was treated with ALA, while PpIX was also increased markedly when ALA and EDTA were added simultaneously. A bactericidal effect on P. aeruginosa was not observed when PDT was conducted using ALA alone, although a bacteriostatic effect was observed after treatment with both ALA and EDTA.The optimal LED wavelength for PDT was 410 nm. Further investigations are needed to determine whether PDT combined with ALA and EDTA treatment has a bacteriostatic effect in vivo.
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Report
(3 results)
Research Products
(2 results)
