study of voltage-applied unfrozen storage of tissue

Research Project

Project/Area Number	25861711
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Plastic surgery
Research Institution	Kansai Medical University
Principal Investigator	kuro atsuyuki 関西医科大学, 医学部, 助教 (20509492)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	氷点下非凍結保存 / 組織保存 / 再接着 / 切断指 / マイクロサージャリー / 氷感庫
Outline of Final Research Achievements	Study of voltage-applied unfrozen storage of tissue. Rat femoral artery was preserved under the conditions of 4 ℃ 0V and -2 ℃ 1000V. It was observed vascular endothelial cells in a transmission electron microscope. Endothelial cells were dissected under the conditions of 4 ℃ 0V in 3 days. The viability of the blood vessel for 3 days preserved rat aorta using a CCK that can measure cell viability was assessed. -2 ℃ 1000V conditions were significantly survival. 3 days preserved rat femoral artery was performed bypass graft to the same strain rats. Good trend at -2 ℃ 1000V conditions came.

Report

(4 results)