study of voltage-applied unfrozen storage of tissue
| Project/Area Number |
25861711
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
kuro atsuyuki 関西医科大学, 医学部, 助教 (20509492)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 氷点下非凍結保存 / 組織保存 / 再接着 / 切断指 / マイクロサージャリー / 氷感庫 |
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| Outline of Final Research Achievements |
Study of voltage-applied unfrozen storage of tissue. Rat femoral artery was preserved under the conditions of 4 ℃ 0V and -2 ℃ 1000V. It was observed vascular endothelial cells in a transmission electron microscope. Endothelial cells were dissected under the conditions of 4 ℃ 0V in 3 days. The viability of the blood vessel for 3 days preserved rat aorta using a CCK that can measure cell viability was assessed. -2 ℃ 1000V conditions were significantly survival. 3 days preserved rat femoral artery was performed bypass graft to the same strain rats. Good trend at -2 ℃ 1000V conditions came.
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Report
(4 results)
Research Products
(1 results)
