Photodynamic therapy for methicillin-resistant staphylococcus aureus infected burn wound

Research Project

Project/Area Number	25861726
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Emergency medicine
Research Institution	Osaka City University
Principal Investigator	MORIMOTO Kuniyuki 大阪市立大学, 医学(系)研究科(研究院), 医員 (10648562)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	光線力学療法 / MRSA / 5-アミノレブリン酸 / 熱傷
Outline of Final Research Achievements	An in vitro study, total porphyrin production in MRSA was increased three times from P. aeruginosa when 5-ALA was added, and 16% were protoporphirin 9 in quantity of porphyrin. It decreased from 16% to 11% in the MRSA whereas PpⅨ rose 7 times in P. aeruginosa when ALA and EDTA were added simultaneously. This result shows administration of 5-ALA alone was effective without adding EDTA in PDT for MRSA. An in vivo study, we demonstrated that PpⅨ successfully accumulated in MRSA on the ulcer surface after intraperitoneal administration of 5-ALA to mice. Furthermore, we found that ALA-PDT accelerated wound healing. Our findings indicate that ALA-PDT may be a new treatment option for MRSA-infected wound.