Elucidation of pathological condition of sepsis by using iPS cells for creation of novel therapy

Research Project

Project/Area Number	25861727
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Emergency medicine
Research Institution	Nara Medical University
Principal Investigator	KASUDA Shogo 奈良県立医科大学, 医学部, 助教 (70434941)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	敗血症 / sphingosine-1-phosphate / 血管内皮細胞 / iPS細胞 / Sphingosine-1-phosphate
Outline of Final Research Achievements	iPS cells differentiated into hematopoietic embryoid body (EB) in order to establish novel therapy for sepsis. We revealed that EB protected the endothelial integrity by producing Sphingosine-1-phosphate (S1P). Intravenous injection of EB into sepsis mice significantly restored their survival rates compared to saline-injected mice. Also, onset of lung edema was protected by EB injection. S1P produced from EB exerted protective effects on endothelial integrity of lungs, resulting in inhibition of sepsis progression.

Report

(3 results)