| Project/Area Number |
25861730
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Iwate Medical University |
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Principal Investigator |
KAN SHIGENORI 岩手医科大学, 医学部, 助教 (80633081)
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| Co-Investigator(Renkei-kenkyūsha) |
INADA Katuya 岩手医科大学, 医学部, 助教授 (80048446)
TAKAHASHI Gaku 岩手医科大学, 医学部, 助教 (60453304)
TANPO Akihito 岩手医科大学, 医学部, 助教 (80531524)
KOJIKA Masahiro 岩手医科大学, 医学部, 助教 (40347878)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 感染症 / 敗血症 / エンドトキシン / 多白血球血漿 / HES / PMX－DHP / グラム陰性桿菌 / Leucocyte Rich Plasma |
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| Outline of Final Research Achievements |
We focused our attention on the endotoxin present within and on the surface of white blood cells and attempted to establish a new sample preparation method for endotoxin assays in leukocyte-rich plasma (LRP), taking advantage of the erythrocyte-aggregating property of hydroxyethyl starch (HES). We used an endotoxin-specific turbidimetric kinetic assay, which is the conventional method used to assay endotoxin levels in platelet-rich plasma (PRP). Then, we comparatively assessed the assay results obtained with the endotoxin assay using PRP and LRP. It was found that the sensitivity of endotoxin assay in the diagnosis of infections caused by Gram-negative bacteria. These results suggest that our newly developed leukocyte-rich plasma endotoxin assay may contribute to an improvement in the rate of sepsis diagnosis.
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