Suppression of IL-6 gene and protein expression by PEEP-like stretching of human pulmonary artery endothelial cells
| Project/Area Number |
25861731
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | VILI / 遺伝子発現解析 / 伸展刺激 / PEEP / 遺伝子発現 |
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| Outline of Final Research Achievements |
The time courses of interleukin (IL)-6 gene expression and protein production were examined in human pulmonary artery endothelial cells (HPAECs) subjected to stretching comprising excessive elongation and slight elongation (excessive plus PEEP-like stretching). The findings that the peak value of IL-6 gene expression induced by excessive plus PEEP-like stretching was lower than that induced by excessive stretching, and that expression of the protein was suppressed by excessive plus PEEP-like stretching, suggest that PEEP-like stretching reduces IL-6 gene expression and that the small degree of IL-6 gene induction is insufficient for synthesis of IL-6 protein. Thus, PEEP-like cyclic stretching suppresses the expression of both the IL-6 gene and protein.
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Report
(4 results)
Research Products
(1 results)
