Suppression of IL-6 gene and protein expression by PEEP-like stretching of human pulmonary artery endothelial cells

Research Project

Project/Area Number	25861731
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Emergency medicine
Research Institution	Kitasato University
Principal Investigator	KOBAYASHI KOZUE 北里大学, 医療衛生学部, 助教 (60448975)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	VILI / 遺伝子発現解析 / 伸展刺激 / PEEP / 遺伝子発現
Outline of Final Research Achievements	The time courses of interleukin (IL)-6 gene expression and protein production were examined in human pulmonary artery endothelial cells (HPAECs) subjected to stretching comprising excessive elongation and slight elongation (excessive plus PEEP-like stretching). The findings that the peak value of IL-6 gene expression induced by excessive plus PEEP-like stretching was lower than that induced by excessive stretching, and that expression of the protein was suppressed by excessive plus PEEP-like stretching, suggest that PEEP-like stretching reduces IL-6 gene expression and that the small degree of IL-6 gene induction is insufficient for synthesis of IL-6 protein. Thus, PEEP-like cyclic stretching suppresses the expression of both the IL-6 gene and protein.