The study of blood clotting and venous repair regulated by a novel protein

• Project/Area Number: 25861756
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: ASANO Satoshi 広島大学, 医歯薬保健学研究院(歯), 助教 (30570535)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 細胞移動 / PI3-kinase / 細胞骨格 / 創傷治癒 / イノシトールリン脂質 / シグナル伝達 / 血小板増殖因子

Outline of Final Research Achievements

To repair damaged tissues, fibroblasts form lamellipodia at its leading edge and migrate to the damaged tissues, resulting in contribution of wound healing. The lamellipodia formation is regulated by the metabolism of PI(4,5)P2, an inositol phospholipid, into PI(3,4,5)P3. In this study, we revealed a possible regulatory mechanism by phospholipase C-related catalytically inactive protein (PRIP), an inositol phospholipid-binding partner, in PDGF-induced cell migration.

Research Products

• [Journal Article] Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion (2014)
  • Author(s): Satoshi Asano, Tomomi Nemoto, Tomoya Kitayama, Kae Harada, Jun Zhang, Kana Harada, Isei Tanida, Masato Hirata, and Takashi, Kanematsu
  • Journal Title: Biological Open Volume: 3 Issue: 6 Pages: 463-474
  • DOI: 10.1242/bio.20147591
  • Peer Reviewed / Open Access
• [Journal Article] Phospholipase C-Related Catalytically Inactive Protein (PRIP) regulates lipolysis in adipose tissue by modulating the phosphorylation of hormone-sensitive lipase (2014)
  • Author(s): T. Okumura, K. Harada, K. Oue, J. Zhang, S. Asano, M. Hayashiuchi, A. Mizokami, H. Tanaka, M. Irifune, N. Kamata, M. Hirata, and T. Kanematsu.
  • Journal Title: PLoS One Volume: 9 Issue: 6 Pages: e100559-e100559
  • DOI: 10.1371/journal.pone.0100559
  • Peer Reviewed / Open Access
• [Journal Article] Phospholipase C-related catalytically inactive protein participates in the autophagic elimination of Staphylococcus aureus infecting mouse embryonic fibroblasts. (2014)
  • Author(s): Harada-Hada K, Harada K, Kato F, Hisatsune J, Tanida I, Ogawa M, Asano S, Sugai M, Hirata M, Kanematsu T.
  • Journal Title: PLoS One. Volume: 9 Issue: 5 Pages: e98285-e98285
  • DOI: 10.1371/journal.pone.0098285
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Regulation of cell migration by PRIP: PRIP protects PI(4,5)P2 and decreases PDGF-induced PI(3,4,5)P3 synthesis in the plasma membrane (2014)
  • Author(s): Satoshi Asano, Takashi Kanematsu
  • Organizer: 第３７回 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-26 – 2014-11-27
• [Presentation] Regulation of cell migration by phospholipase C-related catalytically inactive protein (2014)
  • Author(s): S. Asano, and T. Kanematsu.
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Regulation of insulin secretion by phospholipase C-related catalytically inactive protein (2013)
  • Author(s): 浅野智志
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山コンベンションセンター
• [Presentation] Phospholipase C-related catalytically inactive protein modulates insulin secretory vesicle movement (2013)
  • Author(s): 浅野智志
  • Organizer: 第５回広島カンファレンス
  • Place of Presentation: 広島国際会議場
• [Presentation] Regulation of second phase of insulin secretion by phospholipase C-related catalytically inactive protein (2013)
  • Author(s): 浅野智志
  • Organizer: 46th meeting of the Hiroshima University Dental Society
  • Place of Presentation: 広島大学