Reactionary dentin formation by alkali-sensing TRP channels

• Project/Area Number: 25861762
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: KIMURA Maki 東京歯科大学, 歯学部, その他 (90582346)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 象牙芽細胞 / アルカリ刺激

Outline of Final Research Achievements

In odontoblasts, alkaline stimuli activate the intracellular Ca2+ signaling pathway via Ca2+ influx from the extracellular space and intracellular Ca2+ release. These high-pH induced Ca2+ mobilizations showed dependence on extracellular Ca2+; TRPA1 channels mediated the Ca2+ influx. The plasma membrane and intracellular high-pH-sensing mechanisms in odontoblasts may play an important role in cellular functions during dentinogenesis induced by calcium hydroxide.

Research Products

• [Journal Article] Odontoblasts as sensory receptors: transient receptor potential channels, pannexin-1, and ionotropic ATP receptors mediate intercellular odontoblast-neuron signal transduction (2015)
  • Author(s): Shibukawa, Y., Sato, M., Kimura, M., Sobhan, U., Shimada, M., Nishiyama, A., Kawaguchi, A., Soya, M., Kuroda, H., Katakura, A., Ichinohe, T., and Tazaki, M.
  • Journal Title: Pflügers Archiv European Journal of Physiology Volume: 467 Issue: 4 Pages: 843-863
  • DOI: 10.1007/s00424-014-1551-x
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] グアヤコールは象牙芽細胞の細胞内Ca2+流入を活性化する (2013)
  • Author(s): 隝田みゆき，津村麻記，佐藤正樹，Sobhan Ubaidus，大多和由美，山下秀一郎，田﨑雅和, 澁川義幸
  • Journal Title: 歯科学報 Volume: 116(6) Pages: 593-598
  • Peer Reviewed
• [Journal Article] Functional expression of TRPM8 and TRPA1 channels in rat odontoblasts. (2013)
  • Author(s): Maki Tsumura, Ubaidus Sobhan, Masaki Sato, Miyuki Shimada, Akihiro Nishiyama, Aya Kawaguchi, Manabu Soya, Hidetaka Kuroda, Masakazu Tazaki, Yoshiyuki Shibukawa
  • Journal Title: PLoS One Volume: 8 Issue: 12 Pages: e82233-e82233
  • DOI: 10.1371/journal.pone.0082233
  • Peer Reviewed
• [Journal Article] Immunolocalization and distribution of functional temperature-sensitive TRP channels in the salivary glands (2013)
  • Author(s): U Sobhan, M Sato, T Shinomiya, M Okubo, M Tsumura, T Muramatsu, M Kawaguchi, M Tazaki, Y Shibukawa
  • Journal Title: Cell and Tissue Research Volume: 354 Issue: 2 Pages: 507-519
  • DOI: 10.1007/s00441-013-1691-x
  • Peer Reviewed
• [Journal Article] 象牙芽細胞におけるtransient receptor potential melastatin subfamily member 8チャネルの発現の検索 (2013)
  • Author(s): 高橋史子，津村麻記，Sobhan Ubaidus, 佐藤正樹，田﨑雅和, 澁川義幸
  • Journal Title: 医学と生物学 Volume: 157(6-2) Pages: 985-990
  • NAID: 40019705529
  • Peer Reviewed
• [Journal Article] Sodium-calcium exchangers in rat trigeminal ganglion neurons. (2013)
  • Author(s): Hidetaka Kuroda, Ubaidus Sobhan, Masaki Sato, Maki Tsumura, Tatsuya Ichinohe, Masakazu Tazaki, Yoshiyuki Shibukawa
  • Journal Title: Molecular Pain Volume: 9 Pages: 22-22
  • DOI: 10.1186/1744-8069-9-22
  • Peer Reviewed
• [Presentation] Ca2+ signaling activated by alkaline environment in rat odontoblasts (2015)
  • Author(s): Kimura Maki, Sato Masaki, Kojima Yuki, Higashikawa Asuka, Nishiyama Akihiro, Satou Ryoichi, Shiozaki Yuuta, Shimada Miyuki, Ogura Kazuhiro, Mochizuki Hiroyuki, Shibukawa Yoshiyuki, Tazaki Masakazu
  • Organizer: 第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Guaiacol activates TRPV3 channels in mouse odontoblast-lineage cells (2015)
  • Author(s): Miyuki Shimada, Maki Kimura, Masaki Sato, Ubaidus Sobhan, Masakazu Tazaki, Yoshiyuki Shibukawa
  • Organizer: 第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Intercellular odontoblast networks via extracellular glutamate (2015)
  • Author(s): Nishiyama Akihiro, Sato Masaki, Kimura Maki, Katakura Akira, Tazaki Masakazu, Shibukawa Yoshiyuki
  • Organizer: 第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Functional expression of P2X7 receptors in odontoblasts (2015)
  • Author(s): Shiozaki Yuuta, Sato Masaki, Kimura Maki, Shibukawa Yoshiyuki, Tazaki Masakazu
  • Organizer: 第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Voltage-dependent calcium influx pathway in rat odontoblasts (2015)
  • Author(s): Kojima Yuki, Higashikawa Asuka, Kimura Maki, Sato Masaki, Ogura Kazuhiro, Mochizuki Hiroyuki, Shibukawa Yoshiyuki, Tazaki Masakazu
  • Organizer: 第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Merkel cells transduce mechanical stimuli and release neurotransmitters (2015)
  • Author(s): Higashikawa Asuka, Kojima Yuki, Kimura Maki, Sato Masaki, Ogura Kazuhiro, Mochizuki Hiroyuki, Shibukawa Yoshiyuki, Tazaki Masakazu
  • Organizer: 第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Ca2+ signaling activated by alkali-sensing mechanisms in rat odontoblasts (2015)
  • Author(s): M. Kimura, M. Sato, A. Nishiyama, Y. Kojima, A. Higashikawa, M. Shimada, K. Ogura, H. Mochizuki, Y. Shibukawa, M. Tazaki
  • Organizer: 93rd General Session & Exhibition of the International Association for Dental Research
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Intercellular odontoblast-odontoblast Ca2+ signaling via extracellular glutamate (2015)
  • Author(s): A. Nishiyama, M. Sato, M. Kimura, A. Katakura, Y. Shibukawa, M. Tazaki
  • Organizer: 93rd General Session & Exhibition of the International Association for Dental Research
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Extracellular H+ activates intra- and trans-cellular calcium signaling in odontoblasts (2015)
  • Author(s): M. Sato, K. Kurasawa, M. Kimura, Y. Kojima, A. Higashikawa, A. Nishiyama, Y. Shiozaki, R. Sato, M. Shimada, K. Ogura, H. Mochizuki, Y. Shibukawa, M. Tazaki
  • Organizer: 93rd General Session & Exhibition of the International Association for Dental Research
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] ラット象牙芽細胞における電位依存性Ca2+透過性チャネル発現の検討 (2014)
  • Author(s): 小島佑貴，東川明日香，木村麻記，佐藤正樹，澁川義幸，田崎雅和
  • Organizer: 第298回東京歯科大学学会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-18 – 2014-10-19
• [Presentation] メルケル細胞は機械刺激を受容し神経伝達をする (2014)
  • Author(s): 東川明日香，小島佑貴，木村麻記，佐藤正樹，小倉一宏，望月浩幸，澁川義幸，田崎雅和
  • Organizer: 第298回東京歯科大学学会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-18 – 2014-10-19
• [Presentation] 象牙芽細胞におけるイオンチャネル型ATP受容体サブタイプ（P2X7）の機能発現 (2014)
  • Author(s): 塩崎雄大，佐藤正樹，木村麻記，澁川義幸，佐藤亨，田崎雅和
  • Organizer: 第298回東京歯科大学学会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-18 – 2014-10-19
• [Presentation] 象牙芽細胞における酸感受性タンパク質の機能検索 (2014)
  • Author(s): 倉澤馨，佐藤正樹，木村麻記，小島佑貴，東川明日香，小倉一宏，望月浩幸，川口綾，西山明宏，塩崎雄大，佐藤涼一，澁川義幸，田崎雅和
  • Organizer: 第298回東京歯科大学学会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-18 – 2014-10-19
• [Presentation] Odontoblast hydrodynamic receptor theory：TRP チャネルによって仲介される象牙質痛覚発現機構 (2014)
  • Author(s): 澁川義幸，佐藤正樹，木村麻記，小島佑貴，東川明日香，小倉一宏，望月浩幸，田崎雅和
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 象牙芽細胞のアルカリ感受性象牙質形成機構の解明 (2014)
  • Author(s): 木村麻記，佐藤正樹，小島佑貴，東川明日香，隝田みゆき，小倉一宏，望月浩幸，田崎雅和，澁川義幸
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 象牙芽細胞における酸・機械刺激誘発性Ca2+流入はamilorideで抑制される (2014)
  • Author(s): 倉澤馨，佐藤正樹，木村麻記，小島佑貴，東川明日香，小倉一宏，望月浩幸，隝田みゆき，澁川義幸，田崎雅和
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] グアヤコールの前投与は象牙芽細胞における低浸透圧刺激誘発性Ca2+流入を抑制する (2014)
  • Author(s): 隝田みゆき，木村麻記，佐藤正樹，田崎雅和，澁川義幸
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] グルタミン酸による象牙芽細胞-象牙芽細胞間シグナル伝達 (2014)
  • Author(s): 西山明宏，佐藤正樹，木村麻記，田崎雅和，片倉朗，澁川義幸
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Merkel細胞は機械刺激を受容し神経伝達を行う (2014)
  • Author(s): 東川明日香，小島佑貴，木村麻記，佐藤正樹，小倉一宏，望月浩幸，澁川義幸，田崎雅和
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 象牙芽細胞におけるアルカリ感受性の検討 (2013)
  • Author(s): 津村麻記，佐藤正樹，Sobhan Ubaidus，児玉紗耶香，隝田みゆき，西山明宏，田﨑雅和, 澁川義幸
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山市
• [Presentation] マウス由来象牙芽細胞における細胞膜伸展受容TRPチャネルとNa+-Ca2+ exchangerの機能連関 (2013)
  • Author(s): 佐藤正樹，津村麻記，Sobhan Ubaidus，児玉紗耶香，隝田みゆき，西山明宏，望月浩幸，小倉一宏，田﨑雅和, 澁川義幸
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山市
• [Presentation] グアヤコールは象牙芽細胞のTRPV3チャネルに作用する (2013)
  • Author(s): 隝田みゆき，津村麻記，佐藤正樹，Sobhan Ubaidus，山下秀一郎，田﨑雅和, 澁川義幸
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山市
• [Presentation] Odontoblast as sensory receptor cell: TRP channels, pannexin 1 and P2X3 receptor coupling mediate sensory transduction in dentin (2013)
  • Author(s): Y Shibukawa, M Sato, M Tsumura, H Kuroda, U Sobhan, M Tazaki
  • Organizer: 11th International Conference on Tooth Morphorogenesis and Differentiation
  • Place of Presentation: France, La Londe Les Maures
• [Presentation] Expression of TRPM8 and TRPA1 channels in rat odontoblasts (2013)
  • Author(s): M Sato, M Tsumura, U Sobhan, M Tazaki, Y Shibukawa
  • Organizer: 11th International Conference on Tooth Morphorogenesis and Differentiation
  • Place of Presentation: France, La Londe Les Maures