Change in infected root canal microflora during the course of root canal therapy

• Project/Area Number: 25861785
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Tohoku University
• Principal Investigator: Hashimoto Kazuhiro 東北大学, 歯学研究科, 大学院非常勤講師 (70646447)
• Research Collaborator: SATO TAKUICHI 新潟大学, 医歯学系, 教授 (10303132)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 歯学 / 細菌 / 感染根管 / 細菌叢 / メタゲノム / PCR / バイオプローラ

Outline of Final Research Achievements

Obligate anaerobes have been reported to comprise the majority of oral biofilm including intracanal-microbiota. The bacterial profiling of infected root canals has been long performed. In the present study, metagenomic analysis was applied to the profiling of microbiota in infected root canals, and detailed components of the microbiota of infected root canals were found utilizing high-speed sequencers without random-cloning.

Research Products

• [Int'l Joint Research] Forsyth Institute(米国)
• [Presentation] Profiling of microbiota from infected root canals with and without marginal leakage using anaerobic culture and molecular biological techniques (16S rRNA gene sequencing). (2015)
  • Author(s): Sato T, Yamaki K, Kawamura Y, Tomida J, Tian L, Ishida N, Takeuchi Y, Hashimoto K, Abiko Y, Matsuyama J, Takahashi N
  • Organizer: The 25th ECCMID (European Congress of Clinical Microbiology and Infectious Diseases)
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2015-04-25
  • Int'l Joint Research
• [Presentation] Cultivable anaerobic microbiota of infected root canals with/without marginal leakage. (2015)
  • Author(s): Yamaki K, Sato T, Ishida N, Tian L, Hashimoto K, Shimauchi H, Takahashi N
  • Organizer: The 93rd IADR General Session & Exhibition
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-03-13
• [Presentation] 口腔フローラのプロファイリング
  • Author(s): 佐藤拓一，橋本紀洋，河村好章
  • Organizer: 第50回日本細菌学会中部支部総会
  • Place of Presentation: 蒲郡
• [Book] Interface Oral Health Science 2014: Innovative Research on Biosis-Abiosis Intelligent (2015)
  • Author(s): Sato T, Kawamura Y, Yamaki K, Ishida N, Tian L, Takeuchi Y, Hashimoto K, Abiko Y, Mayanagi G, Washio J, Matsuyama J, Takahashi N
  • Publisher: Springer