Acquisition of long-term bonding durability due to collagen cross-linked by using the carbodiimide

• Project/Area Number: 25861789
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: TAKAHASHI MASAHIRO 東京医科歯科大学, 歯学部附属病院, 医員 (20613920)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 歯質接着レジン / 耐久性 / 接着耐久性 / 象牙質コラーゲン

Outline of Final Research Achievements

Bonding system between resin composite and dentin was still developing. Deterioration of the bonding interface had clinical problems such as debonding of restoration and discoloration of bonding interface. The purpose of this study were to reinforce dentin and to acquire constitutivve adhesive durability by crosslinking the collagen. In order to crosslink the collagen, a water soluble carbodiimide was used in this study. It was confirmed that carbodiimide can permeate between collagen molecules. It is expected to contribute to the long-term durability of resin composite restorations.

Research Products

• [Journal Article] Initial and long-term bond strengths of one-step self-etch adhesives with silane coupling agent to enamel-dentin-composite in combined situation (2015)
  • Author(s): Mamanee T, Takahashi M, Nakajima M, Foxton RM, Tagami J.
  • Journal Title: Dental Materials Journal Volume: 34 Issue: 5 Pages: 663-670
  • DOI: 10.4012/dmj.2015-050
  • NAID: 130005101713
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of endogenous human dentin MMPs by Gluma (2014)
  • Author(s): Sabatini C, Scheffel DL, Scheffel RH, Agee KA, Rouch K, Takahashi M, Breschi L, Mazzoni A, Tjäderhane L, Tay FR, Pashley DH.
  • Journal Title: Dental Materials Volume: 7 Issue: 7 Pages: 752-8
  • DOI: 10.1016/j.dental.2014.04.006
  • Peer Reviewed
• [Journal Article] The importance of size-exclusion characteristics of type I collagen in bonding to dentin matrices. (2013)
  • Author(s): Takahashi M, Nakajima M, Tagami J, Scheffel DL, Carvalho RM, Mazzoni A, Cadenaro M, Tezvergil-Mutluay A, Breschi L, Tjäderhane L, Jang SS, Tay FR, Agee KA, Pashley DH.
  • Journal Title: Acta Materialia Volume: 9 Issue: 12 Pages: 9522-9528
  • DOI: 10.1016/j.actbio.2013.07.037
  • Peer Reviewed
• [Presentation] Scrubbing effect of one-step self-etch adhesives on dentin bonding (2016)
  • Author(s): Ornnicha Thanatvarakorn, Taweesak Prasansuttiporn, Masahiro Takahashi, Suppason Thittaweerat, Junji Tagami, Masatoshi Nakajima.
  • Organizer: 45th AADR
  • Place of Presentation: Los Angeles(USA)
  • Year and Date: 2016-03-18
  • Int'l Joint Research
• [Presentation] Effect of universal primer/thermocycling on bond strength to ceramics. (2016)
  • Author(s): Noda Yukari, Takahashi Masahiro, mamanee Teerapong, Nakajima Masatoshi, Ikeda Masaomi, Takagaki Tomohiro, Hosaka Keiichi, Tagami Junji.
  • Organizer: 45th AADR
  • Place of Presentation: Los Angeles(USA)
  • Year and Date: 2016-03-16
  • Int'l Joint Research
• [Presentation] 各種飲食物が歯の着色に及ぼす影響 (2015)
  • Author(s): 荒岡大輔,高橋真広,保坂啓一,大槻昌幸,田上順次
  • Organizer: 日本歯科審美学会学術大会(第26回)
  • Place of Presentation: 東京歯科大学（東京）
  • Year and Date: 2015-11-22
• [Presentation] Bond Strengths of Dual-Cure Adhesive Resin Cements to Dentin. (2015)
  • Author(s): Nakajima M, Takahashi M, Mamanee T, Thitthaweerat S, Seki N, Hosaka K, Tagami J.
  • Organizer: Academy of Dental Materials 2015 Annual Meeting
  • Place of Presentation: Lahaina(USA)
  • Year and Date: 2015-10-08
  • Int'l Joint Research
• [Presentation] 1ステップセルフエッチングシステムの根管象牙質接着性能に対する温風エアブローの効果 (2015)
  • Author(s): 田口敬太，畑山貴志，佐藤健人，吉峰斉昭，高橋真広，保坂啓一，中島正俊，田上順次.
  • Organizer: 第142回日本歯科保存学会
  • Place of Presentation: 北九州国際会議場および 西日本総合展示場（北九州）
  • Year and Date: 2015-06-25
• [Presentation] ユニバーサル表面処理材に対する光照射の有無が レジンセメントの接着性能に及ぼす影響. (2015)
  • Author(s): 荒岡 大輔,佐藤 健人,高橋 真広,池田 正臣,保坂 啓一,中島 正俊,田上 順次.
  • Organizer: 第142回日本歯科保存学会
  • Place of Presentation: 北九州国際会議場および 西日本総合展示場（北九州）
  • Year and Date: 2015-06-25
• [Presentation] ＭＩとアンチエイジングを主題としたダイレクトベニア修復 (2014)
  • Author(s): 保坂啓一，高橋真広 ，池田正臣，中島正俊 ，大槻昌幸 ， 田上順次
  • Organizer: 第25回日本歯科審美学会学術大会
  • Place of Presentation: 高松国際ホテル （高松）
  • Year and Date: 2014-11-22
• [Presentation] 新規レジンセメントRely X Ultimate の象牙質接着強さに及ぼすコンタクトキュアの影響 (2014)
  • Author(s): 高橋真広、ママニーティラポン、千葉彩香、保坂啓一、中島正俊、田上順次
  • Organizer: 日本歯科保存学会 2014 年度春季学術大会(第 140 回)
  • Place of Presentation: 滋賀県立芸術劇場（滋賀）
  • Year and Date: 2014-06-20
• [Presentation] 化学重合型ボンディング材を応用した新規デュアルキュア型レジンセメント「エステセム」の象牙質接着強さに及ぼ す光照射の影響 (2014)
  • Author(s): ママニーティーラポン、高橋真広、千葉彩香、保坂啓一、中島正俊、田上順次
  • Organizer: 日本歯科保存学会 2014 年度春季学術大会(第 140 回)
  • Place of Presentation: 滋賀県立芸術劇場（滋賀）
  • Year and Date: 2014-06-20
• [Presentation] 各種セラミックスへのレジンセメントの接着強さに及ぼす表面処理材の影響 (2014)
  • Author(s): 野田有佳里、高橋真広、ママニーティーラポン、中島正俊、池田正臣、高垣智博、保坂啓一、田上順次
  • Organizer: 日本歯科保存学会 2014 年度春季学術大会(第 140 回)
  • Place of Presentation: 滋賀県立芸術劇場（滋賀）
  • Year and Date: 2014-06-19
• [Presentation] The size-exclusion characteristics of type I collagen in dentin matrices
  • Author(s): Takahashi M, Hosaka K, Nakajima M, Tagami J, Scheffel DLS, Carvalho RM, Mazzoni A, Carrilho MRO, Tezvergil-Mutluay A, Breschi L, Tjaderhane L, Tay FR, Pashley DH
  • Organizer: IADR-APR
  • Place of Presentation: Bangkok (Thailand)
• [Presentation] Dentin Bond Strength and Mechanical Properties of Two-step Self-etch Adhesives
  • Author(s): Sato K, Hosaka K, Chiba A, Thitthaweera S, Takahashi M, Nakajima M, Otsuki M, Tashiro H, Komada W, Miura H, Tagami J
  • Organizer: IADR-APR
  • Place of Presentation: Bangkok (Thailand)
• [Presentation] Bond strength of self-etch adhesives to combined enamel-dentin-composite substrates
  • Author(s): Mamanee T, Takahashi M, Nakajima M, Tagami J.
  • Organizer: IADR-APR
  • Place of Presentation: Bangkok (Thailand)
• [Presentation] Ultra-high-speed Fracture Observation in Resin-dentin Interface under Microtensile Load.
  • Author(s): Hosaka K, Takahashi M, Nakajima M, Nishitani Y, Pashley DH, Tagami J.
  • Organizer: 5th IAD
  • Place of Presentation: Philadelphia (USA)
• [Presentation] 超高速度撮影技術を用いたレジン象牙質接着破壊の可視化
  • Author(s): 保坂啓一, 田代浩史, 佐藤健人, 畑山貴志, 千葉彩香, 高橋真広, 中島正俊,田上順次.
  • Organizer: 第32回日本接着歯学会
  • Place of Presentation: 福岡県歯科医師会館（福岡）