Development of multipurpose medium for guiding multiple cells toward different cell lineages using differential evolution algorithm.

• Project/Area Number: 25861904
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: HONDA Yoshitomo 大阪歯科大学, 歯学部, 講師 (90547259)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 組織幹細胞 / 成長因子 / 骨芽細胞 / 血管内皮細胞 / アルゴリズム / 分化制御 / 骨再生 / 血管 / 差分進化 / 細胞培養 / 培地 / 幹細胞

Outline of Final Research Achievements

Despite the rapid advance of the cell culturing technique, controlling multiple cell types toward the different lineages at the individual culture dish is still challenge in the regenerative medicine. The goal of this project was to construct the basics of multipurpose medium, which can control the cell fate of different cell types at the same time in the same medium by using feedback system control technique (combinatory technique of information engineering and cell biology). As a result, we accumulated findings regarding the effect of ingredients against the cells used in this project. Additionally, we found that dediffrentiated fat cells and some ingredients would be applicable for the project.

Research Products

• [Journal Article] Supplementation of Strontium to a Chondrogenic Medium Promotes Chondrogenic Differentiation of Human Dedifferentiated Fat Cells (2015)
  • Author(s): Okita, N. Honda Y, Kishimoto N, Liao W, Azumi E, Hashimoto Y, Matsumoto N
  • Journal Title: Tissue Eng Part A Volume: 9-10 Issue: 9-10 Pages: 1695-1704
  • DOI: 10.1089/ten.tea.2014.0282
  • Peer Reviewed
• [Journal Article] Application of green tea catechin for inducing the osteogenic differentiation of human dedifferentiated fat cells in vitro. (2015)
  • Author(s): Kaida K, Honda Y, Hashimoto Y, Tanaka M, Baba S.
  • Journal Title: Int J Mol Sci Volume: 16 Issue: 12 Pages: 27988-28000
  • DOI: 10.3390/ijms161226081
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ストロンチウム含有培地による脱分化脂肪細胞の軟骨分化誘導 (2016)
  • Author(s): 本田 義知, 沖田 直也, 安積 瑛子, 岸本 直隆, 橋本 典也, 松本 尚之, 清水谷 公成
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場 (大阪市)
  • Year and Date: 2016-03-18
• [Presentation] 骨再生医療に向けた人工知能応用 (2016)
  • Author(s): 本田 義知
  • Organizer: バイオインテグレーション学会主催教育研究講習会
  • Place of Presentation: 大阪歯科大学 創立100周年記念館 （大阪市）
  • Year and Date: 2016-03-12
  • Invited
• [Presentation] 茶カテキン（エピガロカテキンガレート）によるヒト脱分化脂肪細胞の骨芽細胞分化 (2015)
  • Author(s): 海田 浩治, 本田 義知, 橋本 典也, 馬場 俊輔, 川添 堯彬
  • Organizer: 日本口腔インプラント学会 第35回近畿北陸支部学術大会
  • Place of Presentation: 赤羽ホール （金沢市）
  • Year and Date: 2015-12-13
• [Presentation] ヒト脱分化脂肪細胞の骨芽細胞分化誘導にむけたIn vitroでの緑茶カテキンの応用 (2015)
  • Author(s): 海田 浩治, 本田 義知, 橋本 典也, 田中 昌博, 馬場 俊輔
  • Organizer: 第37回 日本バイオマテリアル学会
  • Place of Presentation: 京都テルサ （京都市）
  • Year and Date: 2015-11-09
• [Presentation] Difference in cellular response of mesenchymal stem cells demonstrated using new osteogenic media (2014)
  • Author(s): 本田義知、今井弘一、橋本典也、武田昭二
  • Organizer: 日本組織培養学会第87回大会
  • Place of Presentation: 東京 星陵会館（東京都千代田区）
  • Year and Date: 2014-05-30