Basic study on novel bone regeneration medicine using a periodontal ligament cell sheet cultured on an amniotic membrane
| Project/Area Number |
25861966
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Amemiya Takeshi 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90398389)
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| Research Collaborator |
Honjo Ken-ichi 京都府立医科大学, 医学部附属病院, 専攻医 (00756877)
KUMAMOTO Sonoko 京都府立医科大学, 医学部附属病院, 専攻医 (90613563)
ADACHI Tetsuya 京都府立医科大学, 医学部附属病院, 専攻医 (10613573)
YAMAMOTO Kenta 京都府立医科大学, 医学研究科, 助教 (00636160)
NAKAMURA Takahiro 京都府立医科大学, 医学研究科, 准教授 (30411078)
YAMAMOTO Toshiro 京都府立医科大学, 医学研究科, 講師 (40347472)
KANAMURA Narisato 京都府立医科大学, 医学研究科, 准教授 (70204542)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 移植・再生医学 / 再生医学 / 細胞・組織 / 歯学 / 臨床 / 移植・再生医療 |
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| Outline of Final Research Achievements |
The bone differentiation of periodontal ligament cells (PDL cells) was induced on an amniotic membrane. PDL cells grown on the amniotic membrane formed a single cell sheet, with the expressions of cell proliferation, mesenchymal cell, and osteoblast markers, intercellular adhesion molecules, and basement membrane components detected by immunostaining. The sheet formed a calcified cell substrate when transplanted into a laboratory animal. The sheet cultured on the amniotic membrane had a bone differentiation potential, suggesting its applicability to novel bone regenerative medicine.
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Report
(4 results)
Research Products
(20 results)
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[Presentation] In vivo investigation of the osteogenic potential of human periodontal ligament cell sheet cultured on amniotic membrane.2014
Author(s)
Adachi K, Amemiya T, Honjyo K, Ichioka H, Nishigaki M, Oseko F, Yamamoto T, Kanamura N.
Organizer
96th The American Association of Oral and Maxillofacial Surgeons Meeting, Scientific Sessions and Exhibition
Place of Presentation
Hawaii Convention Center, Honolulu, Hawaii, USA.
Year and Date
2014-09-12
Related Report
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[Presentation] Immunohistochemical study of periosteal-derived cell sheet cultured on amniotic membrane aiming at periodontal tissue regeneration.2014
Author(s)
Amemiya T, Honjyo K, Adachi K, Nishigaki M, Oseko F, Yamamoto T, Kanamura N
Organizer
96th The American Association of Oral and Maxillofacial Surgeons Meeting, Scientific Sessions and Exhibition
Place of Presentation
Hawaii Convention Center, Honolulu, Hawaii, USA.
Year and Date
2014-09-12
Related Report
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[Presentation] Examination of bone differentiation for human dental pulp-derived cells cultured on amniotic membrane.2014
Author(s)
Honjo K, Yamamoto T, Oseko F, Amemiya T, Kita M, Mazda O, Kanamura N.
Organizer
96th The American Association of Oral and Maxillofacial Surgeons Meeting, Scientific Sessions and Exhibition
Place of Presentation
Hawaii Convention Center, Honolulu, Hawaii, USA.
Year and Date
2014-09-12
Related Report
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[Presentation] Cell sheet culture on amnion aiming at periodontal tissue regeneration.2013
Author(s)
Amemiya T, Nakamura T, Honjyo K, Kumamoto S, Adachi K, Nishigaki M, Oseko F, Yamamoto T, Kinoshita S, Kanamura N.
Organizer
2nd Meeting of the International Association for Dental Research Asia Pacific Region
Place of Presentation
Plaza Athenee, Bangkok, Thailand
Related Report
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