| Project/Area Number |
25862011
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
Michikami Ikumi 大阪大学, 歯学研究科(研究院), 助教 (80589771)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 歯の石灰化 / 小胞輸送 / SNARE / SNAREタンパク質 / エナメル芽細胞 / 石灰化 |
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| Outline of Final Research Achievements |
In this study, we focused on membrane trafficking system as a factor that affects calcification of teeth, and the identification of genes related to the vesicular transport in the tooth-forming cells.
The SNARE (Soluble NSF Attachment protein Receptor) proteins are involved in membrane vesicle fusion. We identified Snap23, one of the SNARE family, expression in tooth by qRT-PCR. In order to confirm the expression and localization of Snap23, we performed sectional in situ hybridization on a murine tooth germ. In situ analysis showed that the specific expression of Snap23 in ameloblast. Therefore, we generated the conditional knock-out mice lacking the Snap23 specifically in ameloblast, and analyzed the phenotype of the mice for the purpose of elucidating the mechanism of calcification of teeth. As a result of the micro-CT analysis, the formation of enamel of S23fl / fl ; K14-Cre mice in the first molar was less than that of the control littermates.
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