Research for molecular diagnosis and treatment of cleft palate with Runx signaling

• Project/Area Number: 25862014
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Osaka University
• Principal Investigator: ITOH Shinsuke 大阪大学, 歯学研究科(研究院), 助教 (40633706)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口蓋裂 / Runx遺伝子 / Runx1 / Cbfb / ノックアウトマウス / Runx / 口蓋形成 / 上皮間葉相互作用

Outline of Final Research Achievements

Cleft palate is one of the most frequent congenital malformation. Recent years, the molecular mechanism has been revealed dramatically from studies which used mutant animals. In this study, we analyzed the functional role of Cbfb and Runx1 in the palate formation, using a conditional knockout mice in which Cbfb or Runx1 gene was inactivated in epithelial cells specifically (K14Cre;Cbfbfl/fl, K14Cre;Runx1fl/fl). We found that both conditional knockout mice exhibited cleft palate which results into abnormal fusion between the primary palate and secondary palate. These results strongly suggest that Runx1/Cbfb signaling is essential for normal palate formation.

Research Products

• [Journal Article] Runx/Cbfb signaling regulates postnatal development of granular convoluted tubule in the mouse submandibular gland. (2015)
  • Author(s): Islam MN, Itoh S, Yanagita T, Sumiyoshi K, Hayano S, Kuremoto K, Kurosaka H, Honjo T, Kawanabe N, Kamioka H, Sakai T, Ishimaru N, Taniuchi I, Yamashiro T
  • Journal Title: Dev Dyn. Volume: 244(3) Issue: 3 Pages: 488-496
  • DOI: 10.1002/dvdy.24231
  • Peer Reviewed / Open Access
• [Journal Article] Topical application of lithium chloride on the pulp induces dentin regeneration. (2015)
  • Author(s): Ishimoto K, Hayano S, Yanagita T, Kurosaka H, Kawanabe N, Itoh S, Ono M, Kuboki T, Kamioka H, Yamashiro T.
  • Journal Title: PLoS One Volume: 10 Issue: 3 Pages: 1-12
  • DOI: 10.1371/journal.pone.0121938
  • Peer Reviewed / Open Access
• [Journal Article] Cartilage–specific overexpression of CCN family member 2/connective tissue growth factor (CCN2/CTGF) stimulates insulin-like growth factor expression and bone growth (2013)
  • Author(s): Tomita, N., Hattori, T., Ito, S., Aoyama, E., Yao, M., Yamashiro, T., Takigawa M.
  • Journal Title: PLoS One Volume: 8 Issue: 3 Pages: e59226-e59226
  • DOI: 10.1371/journal.pone.0059226
  • NAID: 120005345532
  • Peer Reviewed
• [Journal Article] CCN family member 2/connective tissue growth factor (CCN2/CTGF) has anti-aging effects that protect articular cartilage from age-related degenerative changes (2013)
  • Author(s): Itoh, S., Hattori, T., Tomita, N., Aoyama, E., Yutani,Y., Yamashiro, T., Takigawa, M.
  • Journal Title: PLoS One Volume: 8 Issue: 8 Pages: e71156-e71156
  • DOI: 10.1371/journal.pone.0071156
  • NAID: 120005345531
  • Peer Reviewed
• [Presentation] 口蓋裂発生におけるRunx/Cbfbシグナリングの関与 (2014)
  • Author(s): 伊藤慎将、三原聖美、黒坂寛、柳田剛志、河野加奈、上岡寛、山城隆
  • Organizer: 第73回日本矯正歯科学会大会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-20 – 2014-10-22
• [Presentation] Parry-Romberg症候群を伴う患者に長期的に矯正治療を行った症例 (2014)
  • Author(s): 菅原康代、伊藤慎将、住吉久美、山本照子、山城隆、上岡寛
  • Organizer: 第73回日本矯正歯科学会大会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-20 – 2014-10-22
• [Presentation] グルコーストランスポーター1異常症における口腔顎顔面領域の特徴的所見 (2014)
  • Author(s): 三原聖美、上松節子、武田理恵子、伊藤慎将、村田有香、山城隆
  • Organizer: 第73回日本矯正歯科学会大会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-20 – 2014-10-22
• [Presentation] ラーセン症候群の1例-顎顔面形態の特徴と早期治療- (2013)
  • Author(s): 伊藤慎将、上岡寛、本城正、山城隆
  • Organizer: 第56回 中四国矯正歯科学会
  • Place of Presentation: 倉敷
• [Presentation] Runx/Cbfbシグナリングはアンドロゲン代謝を介した唾液腺の雌雄二形性発現を制御する (2013)
  • Author(s): 伊藤 慎将、柳田 剛志、山城 隆
  • Organizer: 第55回 歯科基礎医学会
  • Place of Presentation: 岡山
• [Presentation] 長期的成長ホルモン療法を受けた軟骨低形成症患者の頭蓋顔面の成長を追った1治験例 (2013)
  • Author(s): 伊藤 慎将、菅原 康代、上岡 寛、山城 隆
  • Organizer: 第72回 日本矯正歯科学会
  • Place of Presentation: 松本