Development of new pediodontal regeneration therapy with Muse cells from dental pulp
| Project/Area Number |
25862061
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | Tsurumi University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Muse細胞 / 歯髄 / 歯周組織再生 / 自己複製能 / 三胚葉分化能 / SSEA-3 / CD105 / 磁気ビーズ |
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| Outline of Final Research Achievements |
In this research, we tried to establish the method of isolate and culture Muse (Multilineage-differentiating stress enduring) cells from human dental pulp tissue.
Three strains of cultured human dental pulp cells were used. The methods of cell culture, cell sorting, and cluster formation, according to the protocol. In addition, pluripotency were confirmed by RT-PCR.It became clear that can be separated the Muse cells from the human dental pulp cells.
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Report
(4 results)
Research Products
(1 results)
