Development of new pediodontal regeneration therapy with Muse cells from dental pulp

Research Project

Project/Area Number	25862061
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Periodontology
Research Institution	Tsurumi University
Principal Investigator	NAGANO Takatoshi 鶴見大学, 歯学部, 講師 (10386914)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	Muse細胞 / 歯髄 / 歯周組織再生 / 自己複製能 / 三胚葉分化能 / SSEA-3 / CD105 / 磁気ビーズ
Outline of Final Research Achievements	In this research, we tried to establish the method of isolate and culture Muse (Multilineage-differentiating stress enduring) cells from human dental pulp tissue. Three strains of cultured human dental pulp cells were used. The methods of cell culture, cell sorting, and cluster formation, according to the protocol. In addition, pluripotency were confirmed by RT-PCR.It became clear that can be separated the Muse cells from the human dental pulp cells.

Report

(4 results)